Caldwell M, O'Neill M, Earley B, Leonard B
Department of Pharmacology, University College Galway, Ireland.
Eur J Pharmacol. 1994 Aug 1;260(2-3):191-200. doi: 10.1016/0014-2999(94)90337-9.
To assess the effects of the nitric oxide synthase inhibitor NG-Nitro-L-arginine on behavioural, biochemical and histological changes following global ischaemia, the Mongolian gerbil was used. Ischaemia was induced by bilateral carotid occlusion for 5 min. NG-Nitro-L-arginine was administered i.p. at either 1 or 10 mg/kg 30 min, 6, 24, and 48 h after surgery. 5 min bilateral carotid occluded animals were hyperactive 24, 48 and 72 h after surgery. NG-Nitro-L-arginine caused some attenuation in this hyperactivity. The activity of nitric oxide synthase was increased in the cerebellum, brain stem, striatum, cerebral cortex and hippocampus of 5 min bilateral carotid occluded animals. NG-Nitro-L-arginine reversed the increase in nitric oxide synthase activity in all brain regions. Extensive neuronal death was observed in the CA1 layer of the hippocampus in 5 min bilateral carotid occluded animals 96 h after surgery. NG-Nitro-L-arginine significantly protected against the neuronal death of cells in the CA1 layer.
为了评估一氧化氮合酶抑制剂NG-硝基-L-精氨酸对全脑缺血后行为、生化和组织学变化的影响，使用了蒙古沙鼠。通过双侧颈动脉闭塞5分钟诱导缺血。在手术后30分钟、6小时、24小时和48小时，以1或10mg/kg的剂量腹腔注射NG-硝基-L-精氨酸。双侧颈动脉闭塞5分钟的动物在手术后24小时、48小时和72小时表现为活动亢进。NG-硝基-L-精氨酸使这种活动亢进有所减轻。在双侧颈动脉闭塞5分钟的动物的小脑、脑干、纹状体、大脑皮层和海马中，一氧化氮合酶的活性增加。NG-硝基-L-精氨酸逆转了所有脑区一氧化氮合酶活性的增加。在双侧颈动脉闭塞5分钟的动物手术后96小时，在海马的CA1层观察到广泛的神经元死亡。NG-硝基-L-精氨酸显著保护了CA1层细胞的神经元死亡。
