Nagai T, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Int J Immunopharmacol. 1994 Aug;16(8):605-13. doi: 10.1016/0192-0561(94)90133-3.
When BALB/c mice were treated with a Kampo (Japanese herbal) medicine "Sho-seiryu-to" (SST) (2 g/kg, 10 times) orally from 7 days before to 4 days after the infection and infected with mouse-adapted influenza virus A/PR/8/34 by nasal site-restricted infection, replication of the virus in the nasal cavity and spread of the virus to the lung were efficiently inhibited at 5 days after infection in comparison with water-treated mice. However, another Kampo medicine "Kakkon-to" showed no anti-influenza virus activity in the same condition. The antiviral IgA antibody in the nasal and broncho-alveolar washes of the SST treated mice increased significantly in comparison with that of water-treated control. Oral administration of SST (2 g/kg, 18 times) from 7 days before to 13 days after vaccination also significantly augmented serum hemagglutination-inhibiting antibody by nasal inoculation of influenza HA vaccine (5 micrograms/mouse) that was insufficient to induce antiviral antibody. SST did not inhibit the replication of mouse-adapted influenza virus A/PR/8/34 in Madin-Darby canine kidney cells. SST also did not inhibit the influenza virus sialidase activity against sodium p-nitrophenyl-N-acetyl-alpha-D-neuraminate and hemagglutination by mouse-adapted influenza virus A/PR/8/34. SST showed no influence on interferon production in nasal wash of mice at 5 days after the virus infection. These results suggest that SST confers better protection against influenza virus infection through augmentation of production of antiviral IgA antibody but not direct action to the virus, and can be used as an adjuvant to nasally inoculated influenza HA vaccine.
将BALB/c小鼠从感染前7天至感染后4天口服给予汉方（日本草药）药物“小青龙汤”（SST）（2 g/kg，10次），并通过鼻腔局部感染接种适应小鼠的甲型流感病毒A/PR/8/34，与用水处理的小鼠相比，感染后5天时病毒在鼻腔中的复制及病毒向肺部的扩散受到有效抑制。然而，另一种汉方药物“葛根汤”在相同条件下未显示出抗流感病毒活性。与用水处理的对照小鼠相比，SST处理小鼠的鼻腔和支气管肺泡灌洗液中的抗病毒IgA抗体显著增加。从接种疫苗前7天至接种后13天口服给予SST（2 g/kg，18次），也显著增强了通过鼻腔接种流感HA疫苗（5微克/小鼠）诱导的血清血凝抑制抗体，该疫苗单独接种不足以诱导抗病毒抗体。SST不抑制适应小鼠的甲型流感病毒A/PR/8/34在Madin-Darby犬肾细胞中的复制。SST也不抑制流感病毒对对硝基苯基-N-乙酰-α-D-神经氨酸钠的唾液酸酶活性以及适应小鼠的甲型流感病毒A/PR/8/34的血凝作用。SST对病毒感染后5天小鼠鼻腔灌洗液中的干扰素产生没有影响。这些结果表明，SST通过增强抗病毒IgA抗体的产生而非对病毒的直接作用，赋予对流感病毒感染更好的保护作用，并且可作为鼻腔接种流感HA疫苗的佐剂。
