Nagai T, Urata M, Yamada H
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Immunopharmacol Immunotoxicol. 1996 May;18(2):193-208. doi: 10.3109/08923979609052732.
When aged BALB/c mice (approximately 6 months old) were treated with a Kampo (Japanese herbal) medicine "Sho-seiryu-to (SST)" (1 g/kg, 10 times) orally from 7 days before to 4 days after the infection and infected with mouse-adapted influenza virus A/PR/8/34 (H1N1 subtype) by nasal site-restricted infection, replication of the virus in the broncho-alveolar cavity was efficiently inhibited at 5 days after infection in comparison with water-treated mice. The antiviral IgA antibody in the broncho-alveolar wash of the SST treated aged mice increased significantly. When mice (7 weeks old) were administered orally with SST (1 and 2 g/kg, 7 times) from 4 days before to 3 days after the infection and infected with mouse-adapted influenza virus A/Guizhou/54/89 (H3N2 subtype) or B/Ibaraki/2/85, replication of the viruses in the nasal cavity and lung were significantly inhibited at 4 days after infection in comparison with control mice. When mice infected with influenza virus A/Fukuoka/C29/85 (H3N2) before 14 days were secondary infected with A/PR/8 virus and administered orally with SST (1 g/kg, 5 times) from 2 h to 5 days after the secondary infection, replication of the virus in both nasal and broncho-alveolar cavities were significantly inhibited at 5 days after the secondary infection in comparison with water-treated control. Oral administration of SST (1 g/kg, 18 times) from 7 days before to 14 days after vaccination followed by secondary nasal inoculation of influenza HA vaccine (5 micrograms/mouse) at 14 days after the first vaccination significantly augmented nasal antiviral IgA antibody and broncho-alveolar and serum antiviral IgG antibodies. These results suggest that SST is useful for influenza virus infection on aged persons and for cross-protection of subtypes of influenza A viruses and influenza B virus. SST is also useful for the treatment of influenza virus infection on human which has a history of influenza virus infection and/or influenza vaccination.
将6月龄左右的老龄BALB/c小鼠从感染前7天至感染后4天口服给予汉方（日本草药）制剂“小青龙汤（SST）”（1 g/kg，10次），并通过鼻腔局部感染接种鼠适应株甲型流感病毒A/PR/8/34（H1N1亚型），与给予水处理的小鼠相比，感染后5天病毒在支气管肺泡腔中的复制受到有效抑制。SST处理的老龄小鼠支气管肺泡灌洗液中的抗病毒IgA抗体显著增加。将7周龄小鼠从感染前4天至感染后3天口服给予SST（1和2 g/kg，7次），并接种鼠适应株甲型流感病毒A/贵州/54/89（H3N2亚型）或乙型流感病毒B/茨城/2/85，与对照小鼠相比，感染后4天病毒在鼻腔和肺中的复制受到显著抑制。将在14天前感染甲型流感病毒A/福冈/C29/85（H3N2）的小鼠再次接种A/PR/8病毒，并在再次感染后2小时至5天口服给予SST（1 g/kg，5次），与给予水处理的对照相比，再次感染后5天病毒在鼻腔和支气管肺泡腔中的复制均受到显著抑制。从接种疫苗前7天至接种疫苗后14天口服给予SST（1 g/kg，18次），然后在首次接种疫苗后14天再次经鼻腔接种流感HA疫苗（5微克/只），可显著增强鼻腔抗病毒IgA抗体以及支气管肺泡和血清抗病毒IgG抗体。这些结果表明，SST对老年人流感病毒感染以及甲型流感病毒亚型和乙型流感病毒的交叉保护有效。SST对有流感病毒感染史和/或流感疫苗接种史的人类流感病毒感染治疗也有效。
