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Comparison of cord blood and peripheral blood mononuclear cells as targets for viral isolation and drug sensitivity studies involving human immunodeficiency virus type 1.脐血和外周血单个核细胞作为涉及1型人类免疫缺陷病毒的病毒分离和药物敏感性研究靶点的比较
J Clin Microbiol. 1994 Aug;32(8):2000-2. doi: 10.1128/jcm.32.8.2000-2002.1994.
2
Identification of a mutation at codon 65 in the IKKK motif of reverse transcriptase that encodes human immunodeficiency virus resistance to 2',3'-dideoxycytidine and 2',3'-dideoxy-3'-thiacytidine.在编码对2'，3'-双脱氧胞苷和2'，3'-双脱氧-3'-硫代胞苷具有人类免疫缺陷病毒抗性的逆转录酶的IKKK基序中第65密码子处鉴定出一种突变。
Antimicrob Agents Chemother. 1994 Feb;38(2):275-81. doi: 10.1128/AAC.38.2.275.
3
Rapid in vitro selection of human immunodeficiency virus type 1 resistant to 3'-thiacytidine inhibitors due to a mutation in the YMDD region of reverse transcriptase.由于逆转录酶YMDD区域的突变，人免疫缺陷病毒1型对3'-硫代胞苷抑制剂产生快速体外抗性。
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4
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Selective inhibition of human immunodeficiency viruses by racemates and enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]cytosine.顺式-5-氟-1-[2-(羟甲基)-1,3-氧硫杂环戊烷-5-基]胞嘧啶的外消旋体和对映体对人类免疫缺陷病毒的选择性抑制作用
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Characterization of human immunodeficiency viruses resistant to oxathiolane-cytosine nucleosides.对奥沙硫杂环戊烷胞嘧啶核苷耐药的人类免疫缺陷病毒的特性分析
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High-level resistance to (-) enantiomeric 2'-deoxy-3'-thiacytidine in vitro is due to one amino acid substitution in the catalytic site of human immunodeficiency virus type 1 reverse transcriptase.对（-）对映体2'-脱氧-3'-硫代胞苷的高水平体外抗性是由于1型人类免疫缺陷病毒逆转录酶催化位点的一个氨基酸取代。
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8
Genotypic and phenotypic characterization of HIV-1 isolated from patients receiving (--)-2',3'-dideoxy-3'-thiacytidine.从接受（-）-2'，3'-二脱氧-3'-硫代胞苷治疗的患者中分离出的HIV-1的基因和表型特征
Antiviral Res. 1995 Oct;28(2):133-46. doi: 10.1016/0166-3542(95)00044-m.
9
Activities of the four optical isomers of 2',3'-dideoxy-3'-thiacytidine (BCH-189) against human immunodeficiency virus type 1 in human lymphocytes.2',3'-二脱氧-3'-硫代胞苷（BCH-189）的四种光学异构体在人淋巴细胞中对1型人类免疫缺陷病毒的活性。
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Effects of 3'-deoxynucleoside 5'-triphosphate concentrations on chain termination by nucleoside analogs during human immunodeficiency virus type 1 reverse transcription of minus-strand strong-stop DNA.在人类免疫缺陷病毒1型负链强终止DNA逆转录过程中，3'-脱氧核苷5'-三磷酸浓度对核苷类似物链终止的影响。
J Virol. 1996 Feb;70(2):712-20. doi: 10.1128/JVI.70.2.712-720.1996.

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J Antimicrob Chemother. 2017 Aug 1;72(8):2171-2183. doi: 10.1093/jac/dkx118.
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HIV-1 protease codon 36 polymorphisms and differential development of resistance to nelfinavir, lopinavir, and atazanavir in different HIV-1 subtypes.HIV-1 蛋白酶 36 号密码子多态性与不同 HIV-1 亚型中对奈非那韦、洛匹那韦和阿扎那韦耐药性的差异发展。
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Variations in reverse transcriptase and RNase H domain mutations in human immunodeficiency virus type 1 clinical isolates are associated with divergent phenotypic resistance to zidovudine.人类免疫缺陷病毒1型临床分离株中逆转录酶和核糖核酸酶H结构域突变的差异与齐多夫定的不同表型耐药性相关。
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Natural polymorphisms in the human immunodeficiency virus type 2 protease can accelerate time to development of resistance to protease inhibitors.人类免疫缺陷病毒2型蛋白酶的天然多态性可加速对蛋白酶抑制剂产生耐药性的时间。
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9
Differential maintenance of the M184V substitution in the reverse transcriptase of human immunodeficiency virus type 1 by various nucleoside antiretroviral agents in tissue culture.在组织培养中，多种核苷类抗逆转录病毒药物对1型人类免疫缺陷病毒逆转录酶中M184V替代位点的差异性维持作用。
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10
Lamivudine can exert a modest antiviral effect against human immunodeficiency virus type 1 containing the M184V mutation.拉米夫定对含有M184V突变的1型人类免疫缺陷病毒可发挥一定程度的抗病毒作用。
Antimicrob Agents Chemother. 2003 Feb;47(2):747-54. doi: 10.1128/AAC.47.2.747-754.2003.

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Standardized peripheral blood mononuclear cell culture assay for determination of drug susceptibilities of clinical human immunodeficiency virus type 1 isolates. The RV-43 Study Group, the AIDS Clinical Trials Group Virology Committee Resistance Working Group.用于测定临床1型人类免疫缺陷病毒分离株药物敏感性的标准化外周血单核细胞培养试验。RV - 43研究组，艾滋病临床试验组病毒学委员会耐药性工作组。
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In vitro selection of variants of human immunodeficiency virus type 1 resistant to 3'-azido-3'-deoxythymidine and 2',3'-dideoxyinosine.1型人类免疫缺陷病毒对3'-叠氮-3'-脱氧胸苷和2',3'-双脱氧肌苷耐药变异体的体外筛选
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脐血和外周血单个核细胞作为涉及1型人类免疫缺陷病毒的病毒分离和药物敏感性研究靶点的比较

Comparison of cord blood and peripheral blood mononuclear cells as targets for viral isolation and drug sensitivity studies involving human immunodeficiency virus type 1.

作者信息

Salomon H, Belmonte A, Nguyen K, Gu Z, Gelfand M, Wainberg M A

机构信息

Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada.

出版信息

J Clin Microbiol. 1994 Aug;32(8):2000-2. doi: 10.1128/jcm.32.8.2000-2002.1994.

DOI:10.1128/jcm.32.8.2000-2002.1994
PMID:7527427
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263918/
Abstract

We have shown that umbilical cord blood mononuclear cells (CBMC) are at least as sensitive as peripheral blood mononuclear cells (PBMC) for isolation of human immunodeficiency virus type 1 from the PBMC of infected individuals. Viral replication was more efficiently monitored by a p24 antigen capture assay than by a viral reverse transcriptase test, regardless of whether CBMC or PBMC were employed. We also found that CBMC and PBMC yielded similar results with regard to the susceptibility profiles of both wild-type and drug-resistant variants of human immunodeficiency virus type 1 for 3'-azido-3'-deoxythymidine, 2',3'-dideoxycytidine, and the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine. Finally, viruses isolated on CBMC could be routinely grown on PBMC and vice versa.

摘要

我们已经表明，对于从受感染个体的外周血单个核细胞（PBMC）中分离1型人类免疫缺陷病毒，脐带血单个核细胞（CBMC）至少与外周血单个核细胞一样敏感。无论使用CBMC还是PBMC，通过p24抗原捕获试验比通过病毒逆转录酶试验能更有效地监测病毒复制。我们还发现，就1型人类免疫缺陷病毒野生型和耐药变体对3'-叠氮-3'-脱氧胸苷、2',3'-双脱氧胞苷以及2',3'-双脱氧-3'-硫代胞苷的（-）对映体的敏感性而言，CBMC和PBMC产生了相似的结果。最后，在CBMC上分离的病毒可以常规地在PBMC上生长，反之亦然。