Nunes I P, Johannessen A C, Matre R, Kristoffersen T
Department of Periodontology, University of Bergen, Norway.
J Clin Periodontol. 1994 Sep;21(8):526-32. doi: 10.1111/j.1600-051x.1994.tb01168.x.
The distribution of HLA class II (DR, DP, DQ) and Fc gamma R (I, II, III) was analyzed in the epithelia of patients with advanced marginal periodontitis using cryostat sections incubated with monoclonal antibodies (MoAb) against the Langerhans cell (LC) (CD1a) and various subtypes of HLA class II and Fc gamma R, and the indirect immunofluorescence technique. In the oral gingival epithelium (OGE), LC were concentrated subjacent to the connective tissue papillae, while in the pocket epithelium (PE), they were most abundant at the gingival margin. HLA-DP, DQ, and DR stained LC in both OGE and PE. HLA-DQ+ LC were significantly fewer than DP+ and DR+ LC. HLA-DR also stained keratinocytes (KC) in the whole extension of both OGE and PE. HLA-DP was also observed on KC, but not HLA-DQ. Fc gamma R II stained both LC and focal areas of KC. In PE FC gamma R II+ LC were concentrated near the bottom of the pocket, while in the OGE, they were concentrated at the gingival margin. Fc gamma R III was present only on KC, especially in the basal and suprabasal layer. The results indicate that the epithelial cells are actively involved in the development and maintenance of the inflammation of periodontal disease.
采用间接免疫荧光技术，使用针对朗格汉斯细胞（LC）（CD1a）以及HLA II类和FcγR各亚型的单克隆抗体（MoAb）孵育的低温恒温切片，分析晚期边缘性牙周炎患者上皮组织中HLA II类（DR、DP、DQ）和FcγR（I、II、III）的分布情况。在口腔牙龈上皮（OGE）中，LC集中在结缔组织乳头下方，而在牙周袋上皮（PE）中，它们在牙龈边缘最为丰富。HLA-DP、DQ和DR在OGE和PE中均对LC染色。HLA-DQ+ LC明显少于DP+和DR+ LC。HLA-DR在OGE和PE的整个区域也对角质形成细胞（KC）染色。在KC上也观察到HLA-DP，但未观察到HLA-DQ。FcγR II对LC和KC的局部区域均染色。在PE中，FcγR II+ LC集中在牙周袋底部附近，而在OGE中，它们集中在牙龈边缘。FcγR III仅存在于KC上，尤其是在基底层和基底上层。结果表明，上皮细胞积极参与牙周疾病炎症的发生和维持。
