Sister chromatid exchange frequency is elevated and cell proliferation is delayed in bone marrow cells of starved and marginally malnourished rats.

The frequency of sister chromatid exchanges (SCE) and the cell proliferation kinetic were analyzed in bone marrow (BM) cells of Wistar rats subjected to starvation and marginal malnutrition (MN) after 24h of 5-bromodeoxyuridine (BdUr) implantation. SCE were analyzed in a minimum of 18 consecutive second division metaphases and for cell proliferation, 100 consecutive metaphases were analyzed and classified into the first, second and third or subsequent replication cycles. Rats subjected to starvation and MN exhibited significantly higher mean SCE per lymphocyte in bone marrow than the well nourished rats. Further, they also showed a longer proliferation kinetic in BM cells. These observations indicate that starvation and MN per se resulted in greater SCE and prolongation of the cell cycle in experimental animals. On rehabilitation, the cells with high SCE frequency and prolonged cell cycle in both starved and MN rats were comparable to control groups.