衰老与姐妹染色单体交换。VII. 衰老对体内背景姐妹染色单体交换的影响。

Aging and sister chromatid exchange. VII. Effect of aging on background SCE in vivo.

作者信息

Schneider E L, Bickings C K, Sternberg H

出版信息

Cytogenet Cell Genet. 1982;33(3):249-53. doi: 10.1159/000131761.

Abstract

The frequency of background or spontaneous sister chromatid exchanges (SCE) was estimated in young and old rat and mouse bone marrow cell populations by exposing these cells to increasing concentrations of bromodeoxyuridine (BrdU). At the lowest levels of BrdU where SCE could be accurately assessed, there was no significant difference in background SCE between young and old cell populations. Extrapolation to zero BrdU concentration yielded SCE frequencies of approximately 1.0 SCE/cell/cell cycle in both mouse and rat cells.

摘要

通过将年轻和年老大鼠及小鼠的骨髓细胞暴露于浓度不断增加的溴脱氧尿苷（BrdU）中，估算了背景或自发姐妹染色单体交换（SCE）的频率。在能够准确评估SCE的最低BrdU水平下，年轻和年老细胞群体的背景SCE没有显著差异。外推至零BrdU浓度时，小鼠和大鼠细胞的SCE频率均约为1.0次SCE/细胞/细胞周期。