Zou H Q, Tang Z Y, Ye S L
Liver Cancer Institute, Shanghai Medical University.
Zhonghua Yi Xue Za Zhi. 1994 Aug;74(8):474-5, 517-8.
To investigate the expression of mutant p53 protein (mP53) and alpha-fetoprotein (AFP) during hepatocarcinogenesis, we detected immunohistochemically the specimens from 4 cases of normal human liver, 5 of cirrhosis, 5 of adenomatous hyperplasia (AH), and 16 of hepatocellular carcinoma (HCC) (by Edmondson classification. The 4 cases with normal liver showed negative mP53 and AFP. Four of the 5 cirrhosis cases were positive for mP53 and AFP. In 5 AH cases, 4 were positive for mP53 and AFP. In the 4 cases of grade I HCC, 2 were positive for mP53 and AFP. In the 6 cases of grade II HCC, 4 were positive for mP53 and AFP. In the 6 cases of grade III HCC, 1 showed mP53 positive staining but negative AFP, and 2 were negative for mP53 but positive for AFP, while 3 were negative for both mP53 and AFP. The results indicated that the mutation of p53 gene occurred in the early stage of hepatocarcinogenesis, and may be correlated with the initiation of hepatocarcinogenesis, and that mutant p53 protein probably related to the reactivation of AFP gene.
为研究突变型p53蛋白（mP53）和甲胎蛋白（AFP）在肝癌发生过程中的表达，我们采用免疫组化方法检测了4例正常肝组织、5例肝硬化组织、5例腺瘤样增生（AH）组织及16例肝细胞癌（HCC）组织（按Edmondson分类）的标本。4例正常肝组织mP53和AFP均呈阴性。5例肝硬化组织中有4例mP53和AFP呈阳性。5例AH组织中有4例mP53和AFP呈阳性。4例I级HCC中，2例mP53和AFP呈阳性。6例II级HCC中，4例mP53和AFP呈阳性。6例III级HCC中，1例mP53染色阳性但AFP阴性，2例mP53阴性但AFP阳性，3例mP53和AFP均阴性。结果表明，p53基因的突变发生在肝癌发生的早期阶段，可能与肝癌的发生起始有关，且突变型p53蛋白可能与AFP基因的重新激活有关。
