3H标记的四氢生物蝶呤的合成与表征
Synthesis and characterization of 3H-labelled tetrahydrobiopterin.
作者信息
Werner E R, Schmid M, Werner-Felmayer G, Mayer B, Wachter H
机构信息
Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Austria.
出版信息
Biochem J. 1994 Nov 15;304 ( Pt 1)(Pt 1):189-93. doi: 10.1042/bj3040189.
Abstract
We synthesized [3'-3H]-5,6,7,8-tetrahydrobiopterin from [8,5'-3H]guanosine 5'-triphosphate ([8,5'-3H]GTP) using GTP cyclohydrolase (EC 3.5.4.16), 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase (EC 1.1.1.153). After purification by cation-exchange h.p.l.c. a solution of radiochemically pure (> 95%) [3'-3H]-5,6,7,8-tetrahydrobiopterin with a specific activity of 9.2 Ci/mmol was obtained. The product proved well suited for studying the binding of tetrahydrobiopterin to nitric-oxide synthase.
摘要
我们使用GTP环化水解酶（EC 3.5.4.16）、6-丙酮酰四氢蝶呤合酶和蝶酰三谷氨酸还原酶（EC 1.1.1.153），由[8,5'-3H]鸟苷5'-三磷酸（[8,5'-3H]GTP）合成了[3'-3H]-5,6,7,8-四氢生物蝶呤。通过阳离子交换高效液相色谱法纯化后，获得了放射化学纯（>95%）的[3'-3H]-5,6,7,8-四氢生物蝶呤溶液，其比活度为9.2 Ci/mmol。结果证明该产物非常适合用于研究四氢生物蝶呤与一氧化氮合酶的结合。
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