Castellucci M, Theelen T, Pompili E, Fumagalli L, De Renzis G, Mühlhauser J
Department of Anatomy, Technical University, Aachen, Germany.
Cell Tissue Res. 1994 Nov;278(2):283-9. doi: 10.1007/BF00414172.
Proteases and their inhibitors play a pivotal role in developmental and differentiative processes. In the present report we investigated the immunohistochemical localization of alpha 1-antitrypsin, alpha 1-antichymotrypsin and inter-alpha-trypsin inhibitor in first trimester as well as in term human placentas. For this purpose polyclonal antibodies against these serine-protease inhibitors were used. All inhibitors were expressed in the villous syncytiotrophoblast of first and last trimester placentas. Placental fibrinoid was positively stained for alpha 1-antitrypsin and inter-alpha-trypsin inhibitor throughout gestation. alpha 1-Antitrypsin and alpha 1-antichymotrypsin showed a strong immunostaining in the Hofbauer cells (first trimester and full term placentas). Extravillous cytotrophoblast was negative for the three protease inhibitors throughout gestation. The presence of the three inhibitors in the syncytiotrophoblast suggests a role in coagulative, invasive and immunomodulatory processes. Fibrinoid, staining for alpha 1-antitrypsin and inter-alpha-trypsin inhibitor, could also have an important immunoprotective function. The presence of protease inhibitors in the Hofbauer cells suggests an involvement of these cells in villous remodelling and differentiative processes.
蛋白酶及其抑制剂在发育和分化过程中起着关键作用。在本报告中，我们研究了α1 -抗胰蛋白酶、α1 -抗糜蛋白酶和α-胰蛋白酶抑制剂在孕早期以及足月人胎盘中的免疫组织化学定位。为此，使用了针对这些丝氨酸蛋白酶抑制剂的多克隆抗体。所有抑制剂在孕早期和晚期胎盘的绒毛合体滋养层中均有表达。在整个妊娠期，胎盘纤维蛋白样物质对α1 -抗胰蛋白酶和α-胰蛋白酶抑制剂呈阳性染色。α1 -抗胰蛋白酶和α1 -抗糜蛋白酶在霍夫鲍尔细胞（孕早期和足月胎盘）中显示出强烈的免疫染色。在整个妊娠期，绒毛外细胞滋养层对这三种蛋白酶抑制剂均呈阴性。合体滋养层中这三种抑制剂的存在表明它们在凝血、侵袭和免疫调节过程中发挥作用。对α1 -抗胰蛋白酶和α-胰蛋白酶抑制剂呈阳性染色的纤维蛋白样物质也可能具有重要的免疫保护功能。霍夫鲍尔细胞中蛋白酶抑制剂的存在表明这些细胞参与绒毛重塑和分化过程。
