用于区分人类DNA聚合酶α和ε的新型修饰底物
New modified substrates for discriminating between human DNA polymerases alpha and epsilon.
作者信息
Jasko M V, Semizarov D G, Victorova L S, Krayevsky A A, Kukhanova M K
机构信息
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
出版信息
FEBS Lett. 1995 Jan 2;357(1):23-6. doi: 10.1016/0014-5793(94)01319-v.
PMID:7528146
Abstract
Two 2'-deoxynucleoside 5'-alpha-methylenephosphonyl-beta, gamma-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase alpha from human placenta. Meanwhile, they were not recognized by DNA polymerase epsilon and beta of the same origin as well as by reverse transcriptases from human immunodeficiency virus and avian myeloblastosis virus.
摘要
合成了两种2'-脱氧核苷5'-α-亚甲基膦酰基-β,γ-二磷酸酯。它们由人胎盘来源的DNA聚合酶α掺入DNA链中。同时，它们不被相同来源的DNA聚合酶ε和β以及人免疫缺陷病毒和禽成髓细胞瘤病毒的逆转录酶识别。
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