2'-脱氧核苷5'-三磷酸中三磷酸修饰对其针对各种DNA聚合酶的特异性的影响。
Effect of triphosphate modifications in 2'-deoxynucleoside 5'-triphosphates on their specificity towards various DNA polymerases.
作者信息
Martynov B I, Shirokova E A, Jasko M V, Victorova L S, Krayevsky A A
机构信息
State Research Institute of Organic Chemistry and Technology, Moscow, Russia.
出版信息
FEBS Lett. 1997 Jun 30;410(2-3):423-7. doi: 10.1016/s0014-5793(97)00577-2.
Some natural and glycon-modified dNTPs with beta,gamma-pyrophosphate substitution at the triphosphate residue were synthesized and studied to evaluate the effect of these modifications on substrate properties of dNTPs in DNA synthesis catalyzed by human placental DNA polymerases alpha and beta, avian myeloblastosis virus reverse transcriptase, and calf thymus terminal deoxynucleotidyl transferase. Reverse transcriptase proved to be the enzyme least specific to such modifications; the substrate activity of beta,gamma-methylenediphosphonate substituted dTTP and 3'-azido-3'-deoxy-dTTP decreased in the following order: CF2 = CHF > CBr2 > CFMe >> CH2. This order is individual for each DNA polymerase. It is interesting to mention that beta,gamma-CBr2 substituted dTTP is neither a substrate nor an inhibitor of DNA polymerase beta. This specificity distinguishes DNA polymerase beta from other DNA polymerases studied.
合成并研究了一些在三磷酸残基处具有β,γ-焦磷酸取代的天然和糖基修饰的脱氧核苷三磷酸（dNTPs），以评估这些修饰对人胎盘DNA聚合酶α和β、禽成髓细胞瘤病毒逆转录酶以及小牛胸腺末端脱氧核苷酸转移酶催化的DNA合成中dNTPs底物性质的影响。结果表明，逆转录酶对这类修饰的特异性最低；β,γ-亚甲基二膦酸取代的dTTP和3'-叠氮基-3'-脱氧-dTTP的底物活性按以下顺序降低：CF2 = CHF > CBr2 > CFMe >> CH2。这个顺序因每种DNA聚合酶而异。值得一提的是，β,γ-CBr2取代的dTTP既不是DNA聚合酶β的底物也不是其抑制剂。这种特异性使DNA聚合酶β有别于所研究的其他DNA聚合酶。
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