Peillon R, Drouet E B, Bruneau S, Panteix G, Denoyel G A, De Montclos H P
Unité d'infectiologie, Institut Pasteur de Lyon, France.
FEMS Microbiol Lett. 1994 Nov 15;124(1):75-9. doi: 10.1111/j.1574-6968.1994.tb07264.x.
Ribotyping was investigated as a means of distinguishing ten different serotyped reference strains and seven epidemiologically unrelated isolates of Mycobacterium avium-Mycobacterium intracellulare using a labelled 16S rDNA probe. Thirteen restriction enzymes were screened towards an accurate discrimination of strains. Two selected restriction enzymes (SacI and ClaI) enabled us to classify the 17 strains into ten ribotypes with an index of discrimination of 0.897. Typeability and reproducibility of the method reached 100%. The patterns obtained exhibited polymorphism of RE fragments within and outside the 16S rRNA gene and may be useful for epidemiological studies.
