艾滋病患者的鸟分枝杆菌复合群菌株属于通过rRNA分型方法确定的一个同源组。
Mycobacterium avium complex strains from AIDS patients belong to a homogeneous group ascribed by rRNA typing methods.
作者信息
Peillon R, Drouet E, Wilmotte R, Cotte L, Panteix G, de Montclos H
机构信息
Laboratoire de Mycobactériologie, Laboratoire Marcel Mérieux, Lyon, France.
出版信息
Res Microbiol. 1997 Nov;148(8):683-90. doi: 10.1016/S0923-2508(99)80067-9.
16S rRNA RFLP analysis of Mycobacterium avium complex (MAC) strains isolated from 25 AIDS patients led to identification of seven ribotypes. The same ribotype was determined for strains from 19 patients with and without disseminated disease. When isolates representing the seven ribotypes were examined for their internal transcribed spacer (ITS) between the 16S and 23S rRNA gene nucleotide sequence, four different sequences, including a new ITS type, were recovered. All isolates with the most common ribotype belonged to the sequevar Mav-B. When MAC strains from AIDS patients were compared by ITS sequencing and ribotyping, a significant degree of homogeneity was observed. The discriminatory level reached with ribotyping might be useful for grouping isolates from different clinical sources.
对从25名艾滋病患者中分离出的鸟分枝杆菌复合群（MAC）菌株进行16S rRNA限制性片段长度多态性分析，鉴定出7种核糖体类型。19名有播散性疾病和无播散性疾病患者的菌株确定为相同的核糖体类型。当检查代表这7种核糖体类型的分离株16S和23S rRNA基因核苷酸序列之间的内部转录间隔区（ITS）时，获得了4种不同序列，包括一种新的ITS类型。所有具有最常见核糖体类型的分离株均属于序列变种Mav-B。通过ITS测序和核糖体分型对艾滋病患者的MAC菌株进行比较时，观察到高度的同质性。核糖体分型达到的鉴别水平可能有助于对来自不同临床来源的分离株进行分组。
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