Ghassemi M, Andersen B R, Reddy V M, Gangadharam P R, Spear G T, Novak R M
Department of Pathology, University of Illinois, Chicago 60612.
J Infect Dis. 1995 Jan;171(1):68-73. doi: 10.1093/infdis/171.1.68.
Disseminated Mycobacterium avium complex (MAC) infection is an important opportunistic infection in AIDS patients. Because cells of macrophage lineage are targets for both human immunodeficiency virus (HIV) and MAC, the monocytoid cell line U937 was coinfected with both pathogens. Coinfected cultures had increased HIV replication (more than threefold at day 6) and an increased percentage of HIV-infected cells compared with cultures infected only with HIV. The kinetics of HIV replication were significantly increased in this coinfection system as measured by flow cytometry. When cells were infected concurrently, the rate of intracellular growth of MAC was not significantly affected. However, cells preinfected with HIV before infection with MAC showed significant enhancement of MAC growth compared with control cells. The kinetics of cell death were also increased in the coinfection system compared with singly infected controls. Thus, coinfection of monocytoid cells with HIV and MAC in vitro results in reciprocal enhancement of multiplication.
播散性鸟分枝杆菌复合体（MAC）感染是艾滋病患者重要的机会性感染。由于巨噬细胞系细胞是人类免疫缺陷病毒（HIV）和MAC的靶细胞，因此单核细胞系U937细胞被这两种病原体共同感染。与仅感染HIV的培养物相比，共同感染的培养物中HIV复制增加（第6天增加了三倍多），且HIV感染细胞的百分比增加。通过流式细胞术测量，在这种共同感染系统中HIV复制动力学显著增加。当细胞同时被感染时，MAC的细胞内生长速率没有受到显著影响。然而，与对照细胞相比，在感染MAC之前先感染HIV的细胞显示出MAC生长显著增强。与单一感染的对照相比，共同感染系统中的细胞死亡动力学也增加。因此，体外单核细胞与HIV和MAC共同感染导致增殖的相互增强。
