Mancino G, Placido R, Bach S, Mariani F, Montesano C, Ercoli L, Zembala M, Colizzi V
Department of Biology, University of Rome Tor Vergata, Institute of Experimental Medicine, National Research Council, Italy.
J Infect Dis. 1997 Jun;175(6):1531-5. doi: 10.1086/516494.
Mycobacterium tuberculosis and human immunodeficiency virus type 1 (HIV-1) are virulent intracellular pathogens that invade and multiply within macrophages. The effect of M. tuberculosis on HIV-1 infection and replication was analyzed in vitro using human monocyte-derived macrophages (MDM) isolated from peripheral blood mononuclear cells by countercurrent centrifugal elutriation. Preinfection of MDM with M. tuberculosis followed by HIV-1 infection resulted in an increase in p24 release, reverse transcriptase activity, and infective virus production. In contrast, no increase in HIV-1 production was observed when MDM were infected with Mycobacterium avium complex or heat-killed M. tuberculosis. Coinfected MDM were potent stimulators of T cell proliferation, while HIV-1-infected MDM failed to present exogenous tuberculin to T cells. Furthermore, coinfected MDM showed an increased capacity to transmit HIV-1 to activated T cells. These results suggest that M. tuberculosis infection can both up-regulate HIV-1 infection and replication within MDM and increase the efficiency of virus transmission from infected MDM to T cells.
结核分枝杆菌和1型人类免疫缺陷病毒（HIV-1）是毒性很强的细胞内病原体，它们在巨噬细胞内侵入并繁殖。利用通过逆流离心淘析从外周血单核细胞中分离出的人单核细胞衍生巨噬细胞（MDM），在体外分析了结核分枝杆菌对HIV-1感染和复制的影响。先用结核分枝杆菌感染MDM，随后再感染HIV-1，结果导致p24释放、逆转录酶活性和感染性病毒产生增加。相比之下，当用鸟分枝杆菌复合群或热灭活的结核分枝杆菌感染MDM时，未观察到HIV-1产生增加。共感染的MDM是T细胞增殖的强效刺激剂，而感染HIV-1的MDM无法将外源性结核菌素呈递给T细胞。此外，共感染的MDM显示出将HIV-1传播给活化T细胞的能力增强。这些结果表明，结核分枝杆菌感染既能上调MDM内的HIV-1感染和复制，又能提高病毒从感染的MDM传播到T细胞的效率。
