Huber R, Kulka U, Lörch T, Braselmann H, Bauchinger M
GSF-Forschungszentrum für Umwelt und Gesundheit, Institute of Radiobiology, Oberschleissheim/Neuherberg, Germany.
Mutat Res. 1995 Feb;334(1):97-102. doi: 10.1016/0165-1161(95)90035-7.
The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 routinely allows an efficient automatic identification of metaphases not only in lymphocyte preparations, but also in preparations from mammalian cell lines with varying chromosome numbers. Although larger slide areas are required for automated compared to manual metaphase scanning, the automatic system is faster by a factor of about 5. The interactive visual elimination of metaphases of insufficient quality is an easy and fast procedure. METAFER2 allows an unbiased selection of metaphases irrespective of their appearance as homogeneously stained first or harlequin-stained second division cells. Random selection of metaphases is neither influenced by various structural chromosome changes nor by increased frequencies of sister-chromatid exchanges.
在一项常规致突变性试验中，使用经各种前诱变剂处理的非整倍体大鼠肝细胞系，对自动中期查找系统METAFER2的效率进行了评估。比较了通过自动和手动选择中期所生成的数据集。结果表明，METAFER2不仅能在淋巴细胞制备物中，而且能在来自具有不同染色体数目的哺乳动物细胞系的制备物中，常规地实现对中期的高效自动识别。尽管与手动中期扫描相比，自动扫描需要更大的玻片区域，但自动系统的速度要快约5倍。对质量欠佳的中期进行交互式视觉剔除是一个简单快捷的过程。METAFER2能够无偏倚地选择中期，无论它们表现为均匀染色的第一次分裂细胞还是具有斑驳染色的第二次分裂细胞。中期的随机选择既不受各种染色体结构变化的影响，也不受姐妹染色单体交换频率增加的影响。
