Huber R, Lörch T, Kulka U, Braselmann H, Bauchinger M
GSF-National Research Centre for Environment and Health, Institute of Radiobiology, P.O. Box 1129, 85758 Neuherberg, Germany.
Mutat Res. 1998 Nov 9;419(1-3):27-32. doi: 10.1016/s1383-5718(98)00121-1.
Based on the technical configuration of the Metafer2 metaphase finder (Metasystems, Altlussheim, Germany), an automated system was developed that is able to discriminate first/M1 or second cycle/M2 metaphases. For an evaluation of the system in fluorescence plus Giemsa stained preparations of human lymphocytes, a learning sample and an independent test sample was used. Between 8 and 14 separated chromosomes per metaphase were sufficient to achieve correct classification rates between 95 and 100%. For practical application the system can be most efficiently used for biodosimetry of human radiation exposure which requires scoring of thousands of metaphases exclusively in M1. An application for selection of M2 for SCE scoring in mutagenicity testing was possible, but does not provide a comparable advantage.
基于Metafer2中期查找仪（德国阿尔特卢斯海姆的Metasystems公司）的技术配置，开发了一种能够区分第一次/M1或第二次分裂周期/M2中期的自动化系统。为了在人淋巴细胞的荧光加吉姆萨染色制剂中评估该系统，使用了一个学习样本和一个独立测试样本。每个中期有8至14条分离的染色体足以实现95%至100%的正确分类率。对于实际应用，该系统可最有效地用于人类辐射暴露的生物剂量测定，这需要仅对数千个M1中期进行评分。该系统可用于在致突变性测试中选择用于姐妹染色单体交换评分的M2，但没有提供可比的优势。
