Pauksen K, Elfman L, Ulfgren A K, Venge P
Department of Infectious Diseases, Uppsala University, University Hospital Uppsala, Sweden.
Br J Haematol. 1994 Oct;88(2):256-60. doi: 10.1111/j.1365-2141.1994.tb05015.x.
Serum granulocyte-colony stimulating factor (G-CSF) was measured with an ELISA method in patients with acute bacterial and viral infections, or with an atypical pneumonia. Before initiation of antibiotic treatment, G-CSF was found to be significantly increased (799 +/- 1501 ng/l) in sera from 34 patients with an acute bacterial infection compared with the 27 patients with a viral infection (58 +/- 34 ng/l; P < 0.001) and with the eight patients with an atypical pneumonia (60 +/- 33) ng/l; P < 0.001). No significant difference in G-CSF levels was seen between gram-positive and gram-negative bacterial infections. In septic shock, increased G-CSF levels were seen both in patients with leucocytosis and leucopenia. In uncomplicated bacterial infections, both G-CSF and IL-6 were increased on day 0, and decreased rapidly after initiation of antibacterial therapy and before the patients became afebrile. In bacterial infections on day 0, G-CSF levels correlated with mononuclear cells (rs = -0.62, P < 0.001), IL-6 (rs = 0.40, P < 0.05) and S-MPO (rs = -0.5, P < 0.01). In viral infections, G-CSF was correlated with mononuclear cells (rs = 0.41, P < 0.05), white blood cell counts (rs = 0.56, P < 0.01), neutrophils (rs = 0.41, P < 0.05) and CRP (rs = 0.47, P < 0.05). We conclude that G-CSF is rapidly raised in the blood in acute bacterial infections but not in acute viral infections or in infections with Mycoplasma pneumonia. Our results also support the theory that G-CSF is involved in the mechanisms of mobilization of neutrophils into the peripheral circulation.
采用酶联免疫吸附测定法（ELISA）检测急性细菌感染、病毒感染或非典型肺炎患者血清中的粒细胞集落刺激因子（G-CSF）。在开始抗生素治疗前，发现34例急性细菌感染患者血清中的G-CSF显著升高（799±1501 ng/l），而27例病毒感染患者血清中的G-CSF为（58±34 ng/l；P<0.001），8例非典型肺炎患者血清中的G-CSF为（60±33）ng/l；P<0.001）。革兰氏阳性菌和革兰氏阴性菌感染患者的G-CSF水平无显著差异。在感染性休克中，白细胞增多和白细胞减少的患者G-CSF水平均升高。在无并发症的细菌感染中，第0天时G-CSF和白细胞介素-6（IL-6）均升高，在开始抗菌治疗后且患者体温恢复正常前迅速下降。在第0天的细菌感染中，G-CSF水平与单核细胞（rs=-0.62，P<0.001）、IL-6（rs=0.40，P<0.05）和可溶性髓过氧化物酶（S-MPO，rs=-0.5，P<0.01）相关。在病毒感染中，G-CSF与单核细胞（rs=0.41，P<0.05）、白细胞计数（rs=0.56，P<0.01）、中性粒细胞（rs=0.41，P<0.05）和C反应蛋白（CRP，rs=0.47，P<0.05）相关。我们得出结论，急性细菌感染时血液中G-CSF迅速升高，但急性病毒感染或支原体肺炎感染时则不然。我们的结果还支持G-CSF参与中性粒细胞动员至外周循环机制的理论。
