Helbig J H, Lück P C, Witzleb W
Institut für Medizinische Mikrobiologie, Medizinische Akademie, Carl Gustav Carus, Dresden.
Zentralbl Bakteriol. 1994 Jun;281(1):16-23. doi: 10.1016/s0934-8840(11)80632-8.
A panel of monoclonal antibodies was used to serotype 343 Legionella pneumophila isolates, using the indirect immunofluorescence test and ELISA. In addition, the isolates were typed by means of absorbed rabbit antisera to provide a reference procedure. As shown by a comparison of reaction patterns, serogroup-specific monoclonal antibodies were found for serogroups 1, 2, 3, 4, 6, 7, 8, and 10. Monoclonal subtypes were found to exist within serogroups 1, 2, 5, and 6. Using the monoclonal antibody panel introduced for serogroups 1 to 8 and 10, it was possible to serotype or subtype 92% of the isolates tested. The remaining isolates belonged to serogroups 9, 11 to 14 and to a monoclonal subtype of serogroup 5. 8 monoclonal antibodies recognized serogroup-cross-reactive epitopes. Except for serogroups 1, 7, and 11, all others shared a common antigenic determinant. Another common epitope was shared by strains of serogroups 2 and 3. Additional cross-reactivity was associated in particular with strains of serogroups 5, 8, and 10.
采用一组单克隆抗体，通过间接免疫荧光试验和酶联免疫吸附测定法对343株嗜肺军团菌分离株进行血清分型。此外，采用吸收兔抗血清对分离株进行分型，以提供参考方法。通过反应模式比较发现，针对血清群1、2、3、4、6、7、8和10存在血清群特异性单克隆抗体。在血清群1、2、5和6中发现存在单克隆亚型。使用针对血清群1至8和10引入的单克隆抗体组，可对92%的测试分离株进行血清分型或亚型分型。其余分离株属于血清群9、11至14以及血清群5的一个单克隆亚型。8种单克隆抗体识别血清群交叉反应表位。除血清群1、7和11外，所有其他血清群都有一个共同的抗原决定簇。血清群2和3的菌株共有另一个共同表位。额外的交叉反应尤其与血清群5、8和10的菌株有关。
