人类次黄嘌呤磷酸核糖基转移酶（hprt）染色体区域（Xq26）的物理图谱
Physical mapping of the human hprt chromosomal region (Xq26).
作者信息
Lippert M J, Albertini R J, Nicklas J A
机构信息
University of Vermont, VCC Genetics Laboratory, Burlington 05401.
出版信息
Mutat Res. 1995 Jan;326(1):39-49. doi: 10.1016/0027-5107(94)00147-w.
The human hprt chromosomal region (Xq26) was physical-mapped using pulsed field gel electrophoresis (PFGE). This work involved: (i) the recovery of three new genomic DNA markers (DXS1327, DXS1328, and DXS1329), (ii) the ordering of new markers relative to 11 previously available hprt-linked markers by deletion mapping, and (iii) the completion of human T-lymphocyte PFGE Southern blots using the 14 Xq26 markers. A contiguous 1.5-Mb physical map of the region telomeric to hprt was determined. As this map identifies clusters of in vivo unmethylated rare-cutter restriction sites, potential CpG islands are revealed.
利用脉冲场凝胶电泳（PFGE）对人类次黄嘌呤磷酸核糖转移酶（hprt）染色体区域（Xq26）进行了物理图谱绘制。这项工作包括：（i）获得三个新的基因组DNA标记（DXS1327、DXS1328和DXS1329），（ii）通过缺失作图确定新标记相对于11个先前可用的与hprt连锁的标记的顺序，以及（iii）使用14个Xq26标记完成人类T淋巴细胞PFGE Southern印迹分析。确定了hprt端粒区域的一个连续1.5兆碱基的物理图谱。由于该图谱识别出体内未甲基化的稀有切割限制酶位点簇，因此揭示了潜在的CpG岛。
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