Gosset P, Tillie-Leblond I, Janin A, Marquette C H, Copin M C, Wallaert B, Tonnel A B
INSERM U416, Institut Pasteur, Lille, France.
Int Arch Allergy Immunol. 1995 Jan;106(1):69-77. doi: 10.1159/000236892.
The bronchial mucosa of asthmatic patients is characterized by a large influx of eosinophils, monocytes and lymphocytes. Leucocyte migration and accumulation is thought to involve adhesion molecules, as shown in an animal of allergic asthma and in humans with other allergic diseases. The aim of this study was to evaluate the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) on endothelium and epithelium in bronchial biopsies obtained from patients with allergic (n = 17) and non-allergic asthma (n = 18). Bronchial biopsies were taken in asthmatic patients and control subjects (n = 10) by fiberoptic bronchoscopy and embedded in paraffin. The cellular infiltrate was evaluated by May-Grünwald-Giemsa staining. Adhesion molecule expression was analyzed by immunohistochemistry using mouse monoclonal antibodies; the results were expressed as the percentage of positive cells. For each patient, we evaluated the severity of asthma as defined by the AAS score and the treatment. In controls, low expression of ICAM-1 was observed on the epithelium and endothelium (9.6 +/- 2.7 and 11.2 +/- 4.1%, respectively), while E-selectin and VCAM-1 were not expressed. In patients with allergic asthma, a significant increase of ICAM-1 expression was observed on epithelium and endothelium (28 +/- 5.3 and 35.6 +/- 5%, respectively), whereas E-selectin (17.4 +/- 4.8%) and VCAM-1 (12.8 +/- 3.6%) were overexpressed only on endothelium. In allergic asthmatic patients, adhesion molecule expression on endothelium was correlated with eosinophil and total leucocyte infiltrate (p < 0.05). In contrast, adhesion molecule expression in biopsies from patients with non-allergic asthma (14.1 +/- 5.2 and 15.3 +/- 3.6% for ICAM-1 expression on epithelium and endothelium, respectively) was not significantly different from the control subjects.(ABSTRACT TRUNCATED AT 250 WORDS)
哮喘患者的支气管黏膜以大量嗜酸性粒细胞、单核细胞和淋巴细胞流入为特征。如在过敏性哮喘动物和患有其他过敏性疾病的人类中所示，白细胞迁移和聚集被认为涉及黏附分子。本研究的目的是评估从过敏性（n = 17）和非过敏性哮喘（n = 18）患者获得的支气管活检组织中内皮和上皮细胞上E-选择素、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达。通过纤维支气管镜对哮喘患者和对照受试者（n = 10）进行支气管活检，并将其嵌入石蜡中。通过May-Grünwald-Giemsa染色评估细胞浸润情况。使用小鼠单克隆抗体通过免疫组织化学分析黏附分子表达；结果以阳性细胞百分比表示。对于每位患者，我们根据AAS评分和治疗情况评估哮喘的严重程度。在对照组中，上皮和内皮细胞上观察到ICAM-1的低表达（分别为9.6 +/- 2.7%和11.2 +/- 4.1%），而E-选择素和VCAM-1未表达。在过敏性哮喘患者中，上皮和内皮细胞上观察到ICAM-1表达显著增加（分别为28 +/- 5.3%和35.6 +/- 5%），而E-选择素（17.4 +/- 4.8%）和VCAM-1（12.8 +/- 3.6%）仅在内皮细胞上过表达。在过敏性哮喘患者中，内皮细胞上的黏附分子表达与嗜酸性粒细胞和总白细胞浸润相关（p < 0.05）。相比之下，非过敏性哮喘患者活检组织中的黏附分子表达（上皮和内皮细胞上ICAM-1表达分别为14.1 +/- 5.2%和15.3 +/- 3.6%）与对照受试者无显著差异。（摘要截断于250字）
