Hongo M, Miyasaka A, Suzuki F, Hashiba T
Mol Gen Genet. 1994 Nov 1;245(3):265-71. doi: 10.1007/BF00290105.
The plant pathogenic isolate RI-64 of anastomosis group 4 of Rhizoctonia solani possesses three linear DNA plasmids (pRS64-1, -2, and -3). Unique poly(A)- RNA, 0.5 kb in length and hybridizable with the pRS64 DNAs was found in mycelial cells of the isolate RI-64. The overall homology at the nucleotide level between pRS64-1, -2, and -3, and the cDNA prepared from the poly(A)- RNA was 100%, 73%, and 84%, respectively. The open reading frames found in pRS64-1, -2, and -3 (ORF1-1, ORF2-1, and ORF3-1) are 68 amino acids long. The amino acids sequence showed no significant homology with known proteins. Extracts from Escherichia coli cells expressing ORF1-1 contain a specific protein of 7 kDa. Antisera raised against the ORF1-1 product obtained from E. coli cells cross-reacted with the specific proteins found in the mycelia. The results indicate that the DNA plasmids found in R. solani contain a sequence that encodes a specific protein which may be involved in determination of plant pathogenicity.
立枯丝核菌融合群4的植物病原分离株RI - 64含有三个线性DNA质粒（pRS64 - 1、- 2和- 3）。在分离株RI - 64的菌丝体细胞中发现了独特的长度为0.5 kb且可与pRS64 DNA杂交的聚腺苷酸化RNA（poly(A)- RNA）。pRS64 - 1、- 2和- 3与从该聚腺苷酸化RNA制备的cDNA在核苷酸水平上的总体同源性分别为100%、73%和84%。在pRS64 - 1、- 2和- 3中发现的开放阅读框（ORF1 - 1、ORF2 - 1和ORF3 - 1）长度为68个氨基酸。该氨基酸序列与已知蛋白质无明显同源性。表达ORF1 - 1的大肠杆菌细胞提取物含有一种7 kDa的特异性蛋白质。针对从大肠杆菌细胞获得的ORF1 - 1产物产生的抗血清与菌丝体中发现的特异性蛋白质发生交叉反应。结果表明，在立枯丝核菌中发现的DNA质粒含有一个编码特异性蛋白质的序列，该蛋白质可能参与植物致病性的决定。
