Katsura K, Suzuki F, Miyashita S I, Nishi T, Hirochika H, Hashiba T
Laboratory of Plant Pathology, Faculty of Agriculture, Tohoku University, Sendai 981, Japan.
Curr Genet. 1997 Dec;32(6):431-5. doi: 10.1007/s002940050299.
The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. Comparison of the nucleotide sequences among the three plasmid DNAs indicated the presence of four regions with more than 86% sequence homology, suggesting the development of three plasmid DNAs from a common ancestor. A computer-based study of the pRS64-2 DNA-folding at both termini predicted hairpin loop structures. The hairpin loops consisted of the left- and right-hand terminal 113 and 105 nucleotides, respectively, and had no sequence homology. They had not undergone flip-flop inversion. The hairpins form cruciform base-paired structures.
测定了植物致病真菌立枯丝核菌的三个线性DNA质粒之一（pRS64 - 2）的完整核苷酸序列。pRS64 - 2 DNA由2877个核苷酸组成。还测定了另外两个质粒（pRS64 - 1和pRS64 - 3）中间2.2 kb区域的核苷酸序列。三种质粒DNA的核苷酸序列比较表明存在四个序列同源性超过86%的区域，这表明三种质粒DNA是由一个共同祖先演化而来的。基于计算机对pRS64 - 2两端DNA折叠的研究预测了发夹环结构。发夹环分别由左手端和右手端的113个和105个核苷酸组成，且没有序列同源性。它们没有发生翻转倒置。这些发夹形成十字形碱基配对结构。
