大鼠脑一氧化氮合酶在杆状病毒感染昆虫细胞中的表达及纯化酶的特性研究
Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme.
作者信息
Harteneck C, Klatt P, Schmidt K, Mayer B
机构信息
Institut für Pharmakologie, Freie Universität Berlin, Germany.
出版信息
Biochem J. 1994 Dec 15;304 ( Pt 3)(Pt 3):683-6. doi: 10.1042/bj3040683.
Abstract
Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.
摘要
大鼠脑一氧化氮合酶在杆状病毒感染的昆虫细胞中高水平表达，并通过亲和层析纯化至表观均一。该酶的比活性约为1 μmol瓜氨酸·分钟⁻¹·毫克蛋白⁻¹，每摩尔亚基分别含有0.93、0.45、0.18和0.23摩尔的血红素、(6R)-5,6,7,8-四氢-L-生物蝶呤（H4生物蝶呤）、黄素腺嘌呤二核苷酸（FAD）和黄素单核苷酸（FMN）。
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