Santos C L, Giorgi R R, Frochtengarten F, Elias M C, Chammas R, Brentani R R
Ludwig Institute for Cancer Research, São Paulo, Brasil.
Int J Clin Lab Res. 1994;24(3):148-53. doi: 10.1007/BF02592445.
The integrin family of adhesion receptors is likely to be important for tumor cell invasion and dissemination. We have studied the effects of the differentiating agents retinoic acid on integrin expression by the human melanoma cell line MeWo. Our results show that this agent inhibits cellular proliferation, increases melanin content and induces morphological changes in MeWo cells. Functionally, these alterations are associated with an enhanced adhesion to matrix protein vitronectin and higher levels of expression of vitronectin receptor on the cell surface. This is accompanied by increased levels of alpha v integrin mRNA. Although the mechanism by which retinoic acid regulates the expression of vitronectin receptor in MeWo cells needs further examination, this system may represent a good model for understanding the role of this receptor in melanoma progression, as well the molecular basis for retinoic acid therapy in these tumors.
黏附受体的整合素家族可能对肿瘤细胞的侵袭和扩散很重要。我们研究了分化剂视黄酸对人黑色素瘤细胞系MeWo整合素表达的影响。我们的结果表明，这种试剂可抑制细胞增殖，增加黑色素含量并诱导MeWo细胞发生形态变化。在功能上，这些改变与对基质蛋白玻连蛋白的黏附增强以及细胞表面玻连蛋白受体表达水平升高有关。这伴随着αv整合素mRNA水平的增加。尽管视黄酸调节MeWo细胞中玻连蛋白受体表达的机制需要进一步研究，但该系统可能是理解该受体在黑色素瘤进展中的作用以及这些肿瘤中视黄酸治疗的分子基础的良好模型。
