Hanazawa T, Konno A, Kaneko T, Tanaka K, Ohshima H, Esumi H, Chiba T
Department of Otorhinolaryngology, Chiba University School of Medicine, 1-8, Japan.
Brain Res. 1994 Sep 19;657(1-2):7-13. doi: 10.1016/0006-8993(94)90947-4.
An immunohistochemical study was performed to detect the localization of nitric oxide synthase (NOS) in the rat nasal mucosa by light and electron microscopy. NOS-immunoreactive nerve fibers were observed around blood vessels and seromucous glands. They were found in the subepithelial layer and even within the epithelium. But no NOS-immunoreactivity was found in the olfactory neuroepithelium. Electron microscopy showed that NOS-immunoreactive nerve profiles were in close contact with the cytolemma of respiratory epithelial cells and acinar cells of seromucous glands. NOS-immunoreactive axon varicosities were located at a considerable distance from the smooth muscle of arterioles and small veins as well as the endothelial cells of venules and capillaries. We confirmed that NOS-containing nerves innervated the epithelium, blood vessels and seromucous glands of the nasal mucosa. These findings, collectively, suggested the possibility that nitric oxide participated in the sensory function of the epithelium, the secretory activities of the nasal gland, and the regulation of vascular tone and vascular permeability in the nasal mucosa.
进行了一项免疫组织化学研究，通过光学显微镜和电子显微镜检测大鼠鼻黏膜中一氧化氮合酶（NOS）的定位。在血管和浆液性腺周围观察到了NOS免疫反应性神经纤维。它们存在于上皮下层，甚至上皮内。但在嗅神经上皮中未发现NOS免疫反应性。电子显微镜显示，NOS免疫反应性神经轮廓与呼吸上皮细胞和浆液性腺泡细胞的细胞膜紧密接触。NOS免疫反应性轴突膨体与小动脉和小静脉的平滑肌以及小静脉和毛细血管的内皮细胞相距相当远。我们证实，含NOS的神经支配鼻黏膜的上皮、血管和浆液性腺。这些发现共同提示，一氧化氮可能参与了上皮的感觉功能、鼻腺的分泌活动以及鼻黏膜血管张力和血管通透性的调节。
