Akabayashi A, Zaia C T, Gabriel S M, Silva I, Cheung W K, Leibowitz S F
Rockefeller University, New York, NY 10021.
Brain Res. 1994 Oct 17;660(2):323-8. doi: 10.1016/0006-8993(94)91306-4.
This investigation examined in vivo the relationship between the nucleotide cAMP and hypothalamic levels of two peptides, neuropeptide Y (NPY) and galanin (GAL), which are known to potentiate feeding behavior. In brain-cannulated rats, third ventricular injections of N6,2'-O-dibutyryl cyclic adenosine 3',5'-monophosphate ((Bu)2cAMP, 25 micrograms), compared to saline, caused a significant increase in NPY levels in the arcuate nucleus (ARC) and medial parvocellular portion of the paraventricular nucleus (mPVN), while having no impact in other hypothalamic areas. These site-specific changes in NPY occurred in the absence of any alteration in circulating levels of insulin, corticosterone, aldosterone or glucose, or of changes in hypothalamic levels of GAL. These findings implicate cAMP as having regulatory functions within specific hypothalamic NPY-synthesizing neurons, projecting from the ARC to the mPVN, that are believed to be involved in energy homeostasis.
本研究在体内检测了核苷酸环磷酸腺苷（cAMP）与两种肽（神经肽Y（NPY）和甘丙肽（GAL））的下丘脑水平之间的关系，已知这两种肽会增强摄食行为。在脑插管大鼠中，与注射生理盐水相比，向第三脑室注射N6,2'-O-二丁酰环磷酸腺苷3',5'-单磷酸（（Bu）2cAMP，25微克）会导致弓状核（ARC）和室旁核内侧小细胞部分（mPVN）中NPY水平显著升高，而对其他下丘脑区域没有影响。NPY的这些位点特异性变化发生时，胰岛素、皮质酮、醛固酮或葡萄糖的循环水平没有任何改变，下丘脑GAL水平也没有变化。这些发现表明，cAMP在从ARC投射到mPVN的特定下丘脑NPY合成神经元中具有调节功能，这些神经元被认为参与能量稳态。
