Iwakiri H, Takakusaki K, Nonaka S, Mori S
National Institute for Physiological Sciences, Okazaki, Japan.
Neurosci Lett. 1994 Aug 15;177(1-2):19-22. doi: 10.1016/0304-3940(94)90034-5.
Extracellular levels of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) were measured in the medial pontine reticular formation of acute decerebrate cats. The mean basal levels of 5-HT and 5-HIAA were 26 fmol/20 microliters and 15 pmol/20 microliters. Perfusion of the dialysis probe with high K+ and Ca(2+)-free Ringer solution for 60 min resulted in 4.8-8.5 x increase and 25-48% decrease in the extracellular levels of 5-HT, respectively, in comparison to the basal 5-HT levels. Perfusion with TTX-added Ringer solution for 60 min resulted in a consistent decrease in the extracellular levels of 5-HT.
在急性去脑猫的脑桥内侧网状结构中测量了血清素（5-羟色胺，5-HT）和5-羟吲哚乙酸（5-HIAA）的细胞外水平。5-HT和5-HIAA的平均基础水平分别为26飞摩尔/20微升和15皮摩尔/20微升。与基础5-HT水平相比，用高钾和无钙林格液灌注透析探头60分钟，导致5-HT的细胞外水平分别增加4.8 - 8.5倍和降低25 - 48%。用添加了河豚毒素的林格液灌注60分钟导致5-HT的细胞外水平持续下降。
