Iwakiri H, Matsuyama K, Mori S
Department of Otolaryngology, Asahikawa Medical College, Japan.
Neurosci Res. 1993 Nov;18(2):157-70. doi: 10.1016/0168-0102(93)90018-l.
Extracellular levels of endogenous serotonin (5-hydroxytryptamine; 5-HT) and its major metabolite, 5-hydroxyindoleacetic acid (5-HIAA) were measured in the medial pontine reticular formation (medial PRF) of intact cats. A microdialysis probe was inserted through a guide cannula into the medial PRF. At least 12 h after the probe insertion, in vivo brain microdialysis was initiated. The perfusion rate was 1 microliters/min, and perfusate fractions at regular intervals of 20 min were collected. Changes in extracellular 5-HT levels were compared across sleep-wake states of the animals, such as waking (W), slow wave sleep (SWS) and rapid eye movement (REM) sleep. To assess sleep-wake states, EEG, EMG, EOG and PGO waves were simultaneously recorded in parallel with microdialysis of the medial PRF. Extracellular 5-HT levels were highest (20-28 fmol/20 microliters) during W. As the animals entered SWS, 5-HT levels decreased to about 90% of those during W. The state of REM sleep usually interrupted SWS for 3-8 min. During the longer periods of REM sleep, during the 20 min periods in which the perfusates were collected, we observed the lowest 5-HT levels (60-50%).
在完整猫的脑桥内侧网状结构（内侧PRF）中测量内源性血清素（5-羟色胺；5-HT）及其主要代谢产物5-羟吲哚乙酸（5-HIAA）的细胞外水平。将微透析探针通过引导套管插入内侧PRF。在插入探针至少12小时后，开始进行体内脑微透析。灌注速率为1微升/分钟，每隔20分钟定期收集灌注液部分。比较动物在不同睡眠-觉醒状态下，如清醒（W）、慢波睡眠（SWS）和快速眼动（REM）睡眠时细胞外5-HT水平的变化。为了评估睡眠-觉醒状态，在对内侧PRF进行微透析的同时，并行同步记录脑电图（EEG）、肌电图（EMG）、眼电图（EOG）和脑桥-膝状体-枕叶波（PGO波）。清醒期间细胞外5-HT水平最高（20 - 28飞摩尔/20微升）。当动物进入慢波睡眠时，5-HT水平降至清醒时的约90%。快速眼动睡眠状态通常会打断慢波睡眠3 - 8分钟。在较长时间的快速眼动睡眠期间，在收集灌注液的20分钟时间段内，我们观察到5-HT水平最低（60 - 50%）。
