吕弗勒亚甲蓝染色法：一种检测幽门螺杆菌的廉价且快速的方法。

The Loeffler's methylene blue stain: an inexpensive and rapid method for detection of Helicobacter pylori.

作者信息

Misra V, Misra S P, Dwivedi M, Gupta S C

机构信息

Department of Pathology, Moti Lal Nehru Medical College, Allahabad, India.

出版信息

J Gastroenterol Hepatol. 1994 Sep-Oct;9(5):512-3. doi: 10.1111/j.1440-1746.1994.tb01283.x.

DOI:10.1111/j.1440-1746.1994.tb01283.x

PMID:7530062

Abstract

Loeffler's methylene blue, commonly used as a counter-stain for acid fast bacilli, was used to detect Helicobacter pylori in paraffin sections and touch smears of gastric mucosal biopsies from 15 patients with duodenal ulcer and 35 with non-ulcer dyspepsia, and the results were compared with the modified Giemsa stain. The time taken to stain smears by Loeffler's methylene blue was approximately 10 min and the results correlated well with those stained by the modified Giemsa stain. However, the Loeffler's methylene blue method was found to be simpler, quicker and cheaper than the modified Giemsa stain.

摘要

吕弗勒亚甲蓝常用于抗酸杆菌的复染，本研究用其检测15例十二指肠溃疡患者和35例非溃疡性消化不良患者胃黏膜活检石蜡切片及印片中的幽门螺杆菌，并与改良吉姆萨染色结果进行比较。吕弗勒亚甲蓝染色涂片所需时间约为10分钟，结果与改良吉姆萨染色结果相关性良好。然而，发现吕弗勒亚甲蓝法比改良吉姆萨染色法更简单、快速且成本更低。