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使用罗曼诺夫斯基型（Diff-3）染色法在涂片和组织切片中检测幽门螺杆菌。

Use of Romanowsky type (Diff-3) stain for detecting Helicobacter pylori in smears and tissue sections.

作者信息

Zaitoun A M

机构信息

Pathology Department, Al Qassimi Hospital, Sharjah, United Arab Emirates.

出版信息

J Clin Pathol. 1992 May;45(5):448-9. doi: 10.1136/jcp.45.5.448.

DOI:10.1136/jcp.45.5.448
PMID:1375949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC495314/
Abstract

A Romanowsky type (Diff-3) stain was used for identifying Helicobacter pylori in gastric biopsy specimens from 50 patients with ulcer and non-ulcer dyspepsia. Air dried smears were prepared from fresh biopsy tissue and histological sections were prepared from paraffin wax processed tissue. The Diff-3 technique is accomplished in five steps and takes about 30 seconds. Results using the Diff-3 stain correlated 100% with those using the Giemsa stain. The Diff-3 stain is reliable, simple, rapid, easy and clean, and smears prepared from fresh biopsy tissue can be examined and an immediate report given. The method is recommended for the identification of H pylori in smears prepared from fresh tissue as well as in sections prepared from processed tissue.

摘要

采用罗曼诺夫斯基氏（Diff-3）染色法对50例溃疡和非溃疡性消化不良患者的胃活检标本进行幽门螺杆菌鉴定。从新鲜活检组织制备空气干燥涂片，并从石蜡处理组织制备组织学切片。Diff-3技术分五步完成，耗时约30秒。Diff-3染色结果与吉姆萨染色结果的相关性为100%。Diff-3染色可靠、简单、快速、简便且清洁，从新鲜活检组织制备的涂片可进行检查并立即给出报告。该方法推荐用于从新鲜组织制备的涂片以及从处理组织制备的切片中鉴定幽门螺杆菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e0/495314/34c29a824c65/jclinpath00419-0085-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e0/495314/c8a1520f380e/jclinpath00419-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e0/495314/34c29a824c65/jclinpath00419-0085-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e0/495314/c8a1520f380e/jclinpath00419-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e0/495314/34c29a824c65/jclinpath00419-0085-b.jpg

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本文引用的文献

1
Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.胃炎和消化性溃疡患者胃内不明弯曲杆菌。
Lancet. 1984 Jun 16;1(8390):1311-5. doi: 10.1016/s0140-6736(84)91816-6.
2
Histological identification of Campylobacter using Gimenez technique in gastric antral mucosa.采用吉姆尼兹（Gimenez）技术对胃窦黏膜中的弯曲杆菌进行组织学鉴定。
J Clin Pathol. 1987 Apr;40(4):464-5. doi: 10.1136/jcp.40.4.464.
3
Improved visualisation of mucus penetration by Campylobacter pylori using a Brown-Hopps stain.使用Brown-Hopps染色法改善幽门螺杆菌黏液穿透的可视化。
Clin Microbiol Rev. 1998 Apr;11(2):341-65. doi: 10.1128/CMR.11.2.341.
4
Detection of infection or infectious agents by use of cytologic and histologic stains.利用细胞学和组织学染色检测感染或感染因子。
Clin Microbiol Rev. 1996 Jul;9(3):382-404. doi: 10.1128/CMR.9.3.382.
5
Histology compared with chemical testing for urease for rapid detection of Helicobacter pylori in gastric biopsy specimens.组织学与尿素酶化学检测法在胃活检标本中快速检测幽门螺杆菌的比较。
J Clin Pathol. 1993 Jul;46(7):684-5. doi: 10.1136/jcp.46.7.684.
6
Expression of parathyroid hormone related peptide in human pituitary tumours.甲状旁腺激素相关肽在人垂体肿瘤中的表达。
J Clin Pathol. 1993 Jul;46(7):682-3. doi: 10.1136/jcp.46.7.682.
7
Histological study of chronic gastritis from the United Arab Emirates using the Sydney system of classification.使用悉尼分类系统对来自阿拉伯联合酋长国的慢性胃炎进行组织学研究。
J Clin Pathol. 1994 Sep;47(9):810-5. doi: 10.1136/jcp.47.9.810.
8
The prevalence of lymphoid follicles in Helicobacter pylori associated gastritis in patients with ulcers and non-ulcer dyspepsia.幽门螺杆菌相关性胃炎中淋巴滤泡在溃疡患者和非溃疡性消化不良患者中的患病率。
J Clin Pathol. 1995 Apr;48(4):325-9. doi: 10.1136/jcp.48.4.325.
J Clin Pathol. 1988 Feb;41(2):232. doi: 10.1136/jcp.41.2.232-b.
4
Simple half-gram stain for showing presence of Campylobacter pyloridis in sections.用于在切片中显示幽门弯曲菌存在的简易半克氏染色法。
J Clin Pathol. 1987 Jun;40(6):702. doi: 10.1136/jcp.40.6.702-a.
5
Cresyl fast violet staining method for Campylobacter like organisms.用于类弯曲杆菌的甲酚紫染色法。
J Clin Pathol. 1987 Mar;40(3):353. doi: 10.1136/jcp.40.3.353-b.
6
Simplified techniques for identifying Campylobacter pyloridis.鉴定幽门弯曲菌的简化技术。
J Clin Pathol. 1986 Nov;39(11):1279. doi: 10.1136/jcp.39.11.1279-a.
7
Immunocytochemical detection of Helicobacter pylori in formalin fixed tissue biopsy specimens.福尔马林固定组织活检标本中幽门螺杆菌的免疫细胞化学检测
J Clin Pathol. 1990 Jun;43(6):518. doi: 10.1136/jcp.43.6.518-a.
8
Diagnostic efficiency of an ultrarapid endoscopy room test for Helicobacter pylori.一种用于检测幽门螺杆菌的超快速内镜室检测方法的诊断效率
Gut. 1991 May;32(5):467-9. doi: 10.1136/gut.32.5.467.