鼠伤寒沙门氏菌YG1026和YG1021中检测二苯醚类除草剂的致突变性

Detection of mutagenicity of diphenyl ether herbicides in Salmonella typhimurium YG1026 and YG1021.

作者信息

Oguri A, Karakama K, Arakawa N, Sugimura T, Wakabayashi K

机构信息

Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.

出版信息

Mutat Res. 1995 Jan;346(1):57-60. doi: 10.1016/0165-7992(95)90069-1.

DOI:10.1016/0165-7992(95)90069-1

PMID:7530330

Abstract

Three kinds of diphenyl ether herbicides, 4-nitrophenyl 2,4,6-trichlorophenyl ether (CNP, chlornitrofen), 2,4-dichlorophenyl 3-methoxy-4-nitrophenyl ether (chlomethoxynil) and 2,4-dichlorophenyl 3-methoxycarbonyl-4-nitrophenyl ether (bifenox), were tested for mutagenicity in Salmonella typhimurium YG1026 and YG1021, which have high nitroreductase activity, and also in S. typhimurium TA100 and TA98. CNP and chlomethoxynil showed mutagenicity in S. typhimurium YG1026, without S9 mix, inducing 50 and 304 revertants per microgram. These mutagenicities were suppressed by the addition of S9 mix. CNP and chlomethoxynil were also mutagenic to YG1021 with and without S9 mix, and their mutagenicities were lower than those to YG1026. On the other hand, bifenox was mutagenic to YG1026 only with S9 mix, inducing 3.0 revertants per micrograms. These three herbicides showed no mutagenicity in S. typhimurium TA100 and TA98 either with or without S9 mix.

摘要

对三种二苯醚类除草剂，即4-硝基苯基2,4,6-三氯苯基醚（CNP，除草醚）、2,4-二氯苯基3-甲氧基-4-硝基苯基醚（氯甲醚）和2,4-二氯苯基3-甲氧羰基-4-硝基苯基醚（乙羧氟草醚），在具有高硝基还原酶活性的鼠伤寒沙门氏菌YG1026和YG1021以及鼠伤寒沙门氏菌TA100和TA98中进行了致突变性测试。CNP和氯甲醚在无S9混合物的情况下，对鼠伤寒沙门氏菌YG1026表现出致突变性，每微克分别诱导50个和304个回复突变体。添加S9混合物后，这些致突变性受到抑制。CNP和氯甲醚在有和无S9混合物的情况下，对YG1021也具有致突变性，其致突变性低于对YG1026的致突变性。另一方面，乙羧氟草醚仅在有S9混合物时对YG1026具有致突变性，每微克诱导3.0个回复突变体。这三种除草剂在有或无S9混合物的情况下，对鼠伤寒沙门氏菌TA100和TA98均未表现出致突变性。