Goobar-Larsson L, Luukkonen B G, Unge T, Schwartz S, Utter G, Strandberg B, Oberg B
Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Stockholm, Sweden.
Virology. 1995 Jan 10;206(1):387-94. doi: 10.1016/s0042-6822(95)80054-9.
HIV-1 reverse transcriptase (RT) was found to increase the activity of HIV-1 proteinase in vitro and in eukaryotic cells. The effect of RT on proteinase activity was dose-dependent and independent of pH or salt concentration. The cleavage of sequences corresponding to all the naturally occurring cleavage sites that could be tested in vitro was enhanced. The effect of RT on cleavage was greatest at the cleavage site between RT and integrase. The enhancement of viral proteinase activity by the virus RT may contribute to regulation of the order and/or efficiency of cleavage at different sites during virus replication and maturation.
在体外和真核细胞中均发现，HIV-1逆转录酶（RT）可提高HIV-1蛋白酶的活性。RT对蛋白酶活性的影响呈剂量依赖性，且与pH值或盐浓度无关。在体外可测试的所有天然存在的切割位点对应的序列切割均得到增强。RT对切割的影响在RT与整合酶之间的切割位点处最为显著。病毒RT对病毒蛋白酶活性的增强作用可能有助于调节病毒复制和成熟过程中不同位点切割的顺序和/或效率。
