Sánchez I, San Miguel J F, Corral J, Martín C, Pérez R, González M, Cañizo M C, Orfao A, González-Sarmiento R
Departamento de Medicina, Universidad de Salamanca, España.
Br J Haematol. 1995 Jan;89(1):104-9. doi: 10.1111/j.1365-2141.1995.tb08903.x.
The presence of Ig and TCR gene rearrangement has been reported to occur in ANLL. However, most of the studies have been performed in short series of patients and, in general, not all rearranging genes have been included. We have investigated the configuration of immunoglobulin (Ig) and T-cell receptor loci (TCR) in a series of 160 untreated patients with de novo acute non-lymphoblastic leukaemia (ANLL) and correlated the results with the morphological and immunophenotypic characteristics of blast cells. IGH gene rearrangement was detected in 16/160 cases analysed (10%) and IGK was rearranged in half of them. The incidence of cases displaying TCRB, TCRG and TCRD rearrangements was 5.6%, 13.8% and 13%, respectively. Concomitant recombinatorial events including different Ig and/or TCR genes were frequently detected. Gene rearrangement was not related to the stage of cell differentiation within the myeloid lineage assessed both by morphological and immunophenotypic criteria. Regarding the correlation with the presence of lymphoid related markers, the only relevant association was between the expression of CD7 antigen and TCRG and TCRD gene rearrangement. Our results show that the incidence of gene rearrangement in ANLL may be slightly higher than previously suspected, and that it is not associated with early stages of cell differentiation nor to the expression of lymphoid markers.
据报道，免疫球蛋白（Ig）和T细胞受体（TCR）基因重排存在于急性非淋巴细胞白血病（ANLL）中。然而，大多数研究是在少数患者中进行的，并且一般而言，并非所有重排基因都被纳入研究。我们研究了160例未经治疗的初发急性非淋巴细胞白血病（ANLL）患者的免疫球蛋白（Ig）和T细胞受体基因座（TCR）的构型，并将结果与原始细胞的形态学和免疫表型特征相关联。在分析的160例病例中有16例（10%）检测到IGH基因重排，其中一半同时发生IGK重排。显示TCRB、TCRG和TCRD重排的病例发生率分别为5.6%、13.8%和13%。经常检测到包括不同Ig和/或TCR基因的伴随重组事件。无论是根据形态学还是免疫表型标准评估，基因重排均与髓系细胞分化阶段无关。关于与淋巴系相关标志物存在的相关性，唯一相关的关联是CD7抗原表达与TCRG和TCRD基因重排之间。我们的结果表明，ANLL中基因重排的发生率可能略高于先前的推测，并且它与细胞分化的早期阶段以及淋巴系标志物的表达均无关。
