Eyfjörd J E, Thorlacius S, Steinarsdottir M, Valgardsdottir R, Ogmundsdottir H M, Anamthawat-Jonsson K
Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavik.
Cancer Res. 1995 Feb 1;55(3):646-51.
Abnormalities in the p53 tumor suppressor gene have been shown to affect cell cycle control and lead to genetic instability in cell lines of murine and human origin. We have examined genetic instability in 183 primary human breast carcinomas with and without p53 abnormalities. Mutation analysis was performed by constant denaturant gel electrophoresis and DNA sequencing, and abnormal protein expression was examined by immunohistochemical staining methods. Genetic instability was studied by detection of gene amplification, allelic loss, karyotype analysis, and fluorescent in situ hybridization. We found a significant association between p53 abnormalities and genetic instability detected by these methods.
p53肿瘤抑制基因的异常已被证明会影响细胞周期调控，并导致源自小鼠和人类的细胞系出现基因不稳定。我们检测了183例原发性人类乳腺癌中有无p53异常时的基因不稳定情况。通过恒定变性剂凝胶电泳和DNA测序进行突变分析，并通过免疫组织化学染色方法检测异常蛋白表达。通过检测基因扩增、等位基因缺失、核型分析和荧光原位杂交研究基因不稳定情况。我们发现通过这些方法检测到的p53异常与基因不稳定之间存在显著关联。
