Vedeler C, Ulvestad E, Bjørge L, Conti G, Williams K, Mørk S, Matre R
Department of Neurology, University of Bergen, Norway.
Immunology. 1994 Aug;82(4):542-7.
The expression of CD59, a complement regulator of the formation and function of the terminal cytolytic membrane attack complex, was studied in human normal nervous tissue by immunohistochemical markers using two monoclonal antibodies 1F5 and MEM43. CD59 was present on Schwann cells, neurons and endothelial cells in the peripheral nervous system (PNS), and on Schwann cells in culture. In the central nervous system (CNS) CD59 was found predominantly on endothelial cells. There was also a diffuse staining of white and grey matter of the spinal cord and brain, presumably of microglia, oligodendrocytes, astrocytes and neurons, as these cells were CD59 positive in culture. Furthermore, CD59 was detected in the cerebrospinal fluid (CSF) of healthy individuals. CD59 in the PNS and CNS was glycosyl-phosphatidylinositol linked and had a molecular weight of 19,000-25,000. The presence of CD59 on various cells of the nervous system and in the CSF suggests that regulation of complement activation by this protein is important in neural host defence mechanisms.
利用两种单克隆抗体1F5和MEM43作为免疫组化标记物，在人正常神经组织中研究了终末溶细胞性膜攻击复合物形成和功能的补体调节因子CD59的表达。CD59存在于外周神经系统（PNS）的施万细胞、神经元和内皮细胞上，以及培养的施万细胞上。在中枢神经系统（CNS）中，CD59主要存在于内皮细胞上。脊髓和脑的白质和灰质也有弥漫性染色，推测是小胶质细胞、少突胶质细胞、星形胶质细胞和神经元的染色，因为这些细胞在培养中CD59呈阳性。此外，在健康个体的脑脊液（CSF）中检测到了CD59。PNS和CNS中的CD59通过糖基磷脂酰肌醇连接，分子量为19,000 - 25,000。神经系统各种细胞和CSF中存在CD59表明，该蛋白对补体激活的调节在神经宿主防御机制中很重要。
