Moore T, Bennett M, Kumar V
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1995 Feb 15;154(4):1653-63.
Differentiation of NK cells from pluripotent hematopoietic stem cells is a poorly understood process. Although it is known that NK cells are bone marrow derived and dependent upon an intact bone marrow microenvironment for complete maturation, it is not known if they arise from an intermediate lymphoid stem cell or from progenitors exclusively committed to the NK lineage. To determine whether phenotypically distinct committed NK progenitor cells exist in murine bone marrow, we sorted cells capable of repopulating recipient mice with mature NK cells upon i.v. transfer. We identified a rare population of bone marrow cells with the phenotype Ly6+ Lin- c-kit+ CD43high Fall-3high TSA-1- AA4.1low Rh123high that is highly enriched for the ability to generate NK cells after transplantation. Although these cells are relatively depleted of Rh123low pluripotent stem cells, they are highly enriched for both lymphoid and myeloid repopulating ability. Thus, we have found no evidence to support the existence of a phenotypically distinct transplantable progenitor population in mouse bone marrow that is either exclusively committed to the NK cell lineage or exhibits the functional characteristics of a common lymphoid stem cell.
自然杀伤（NK）细胞从多能造血干细胞分化而来的过程目前了解甚少。虽然已知NK细胞起源于骨髓，并且其完全成熟依赖完整的骨髓微环境，但尚不清楚它们是源自中间淋巴干细胞还是仅源自专门分化为NK细胞系的祖细胞。为了确定在小鼠骨髓中是否存在表型不同的定向NK祖细胞，我们对静脉注射后能够用成熟NK细胞重新填充受体小鼠的细胞进行了分选。我们鉴定出了一群罕见的骨髓细胞，其表型为Ly6+ Lin- c-kit+ CD43high Fall-3high TSA-1- AA4.1low Rh123high，该群体在移植后产生NK细胞的能力方面高度富集。虽然这些细胞相对缺乏Rh123low多能干细胞，但它们在淋巴系和髓系重建能力方面高度富集。因此，我们没有找到证据支持在小鼠骨髓中存在表型不同的可移植祖细胞群体，该群体要么专门分化为NK细胞系，要么具有共同淋巴干细胞的功能特征。
