Rolink A, ten Boekel E, Melchers F, Fearon D T, Krop I, Andersson J
Basel Institute for Immunology, Switzerland.
J Exp Med. 1996 Jan 1;183(1):187-94. doi: 10.1084/jem.183.1.187.
Bone marrow of both normal and rearrangement-deficient mice contains a small population of B220(CD45R)+ cells, which do not express the B lineage marker CD19. Instead, part of this population coexpresses the surface marker CD43 and lacks or expresses very low levels of heat stable antigen (HSA) and BP-1, thus representing a part of Hardy's fraction A (B220(+)-CD43+HSA-, BP-1-) of B lineage development. However, some 20-40% of these B220(+)-CD19- cells also coexpress the NK1.1 surface molecule and do not express genes like VpreB or B29 restricted to the B cell lineage. These cells respond to recombinant interleukin 2 in vitro, and develop into killer cells that can lyse the prototypic NK target tumor cell, YAC-1, as well as syngeneic normal lipopolysaccharide or concanavalin A blasts, providing they lack the surface expression of major histocompatibility complex class I molecules. The implications of these findings for studies on B lymphopoiesis are discussed. It is suggested that the CD19-specific monoclonal antibody is more reliable, as in humans, than B220(CD45R) to detect B lineage cells in mice.
正常小鼠和重排缺陷小鼠的骨髓中都含有一小群B220(CD45R)+细胞，这些细胞不表达B系标志物CD19。相反，这群细胞中的一部分共表达表面标志物CD43，且缺乏或表达极低水平的热稳定抗原（HSA）和BP-1，因此代表了B系发育中Hardy A组分（B220(+)-CD43+HSA-, BP-1-）的一部分细胞。然而，这些B220(+)-CD19-细胞中约20%-40%也共表达NK1.1表面分子，并且不表达诸如VpreB或B29等仅限于B细胞系的基因。这些细胞在体外对重组白细胞介素2有反应，并发育成杀伤细胞，这些杀伤细胞能够裂解典型的NK靶肿瘤细胞YAC-1，以及同基因正常脂多糖或伴刀豆球蛋白A刺激的胚细胞，前提是它们缺乏主要组织相容性复合体I类分子的表面表达。本文讨论了这些发现对B淋巴细胞生成研究的意义。有人提出，在小鼠中检测B系细胞时，CD19特异性单克隆抗体与人类一样，比B220(CD45R)更可靠。
