神经生长因子（NGF）可在体外恢复糖尿病小鼠感觉神经元中降钙素基因相关肽和P物质的耗竭。

Nerve growth factor (NGF) restores depletions of calcitonin gene-related peptide and substance P in sensory neurons from diabetic mice in vitro.

作者信息

Sango K, Verdes J M, Hikawa N, Horie H, Tanaka S, Inoue S, Sotelo J R, Takenaka T

机构信息

Department of Physiology, School of Medicine, Yokohama City University, Japan.

出版信息

J Neurol Sci. 1994 Oct;126(1):1-5. doi: 10.1016/0022-510x(94)90087-6.

DOI:10.1016/0022-510x(94)90087-6

PMID:7530767

Abstract

Dorsal root ganglion neurons from streptozotocin (STZ)-induced diabetic, genetic diabetic and normal mice were cultured in serum-containing media with or without nerve growth factor (NGF). The immunocytochemical analysis carried out after 1 week in culture revealed that the ratios of neurons immunoreactive to calcitonin gene-related peptide (CGRP) in NGF-free medium in the STZ-diabetic mice (average 23.2%) were significantly lower than those in the normal mice (45.1%). The ratios of neurons immunoreactive to CGRP and substance P (SP) in the NGF-free medium were also lower in the genetic diabetic mice (23.6% and 21.8%) than those in the normal ones (40.7% and 34.2%). However, treatment with NGF restored these reduced immunoreactivities in the diabetic groups in a dose-dependent manner. These results show that NGF can be effective for the diabetes-induced depletion of CGRP and SP in sensory neurons, and suggest its possible role in the prevention and improvement of diabetic sensory neuropathy.

摘要

将链脲佐菌素（STZ）诱导的糖尿病小鼠、遗传性糖尿病小鼠和正常小鼠的背根神经节神经元培养于含或不含神经生长因子（NGF）的血清培养基中。培养1周后进行的免疫细胞化学分析显示，在不含NGF的培养基中，STZ诱导的糖尿病小鼠中对降钙素基因相关肽（CGRP）呈免疫反应性的神经元比例（平均23.2%）显著低于正常小鼠（45.1%）。在不含NGF的培养基中，遗传性糖尿病小鼠中对CGRP和P物质（SP）呈免疫反应性的神经元比例（分别为23.6%和21.8%）也低于正常小鼠（分别为40.7%和34.2%）。然而，用NGF治疗可使糖尿病组中这些降低的免疫反应性以剂量依赖的方式恢复。这些结果表明，NGF对糖尿病引起的感觉神经元中CGRP和SP的耗竭可能有效，并提示其在预防和改善糖尿病性感觉神经病变中的可能作用。