Rosner F, Hirshaut Y, Grünwald H W, Deutsch S
Am J Hematol. 1978;5(4):305-14. doi: 10.1002/ajh.2830050405.
Using an in vitro system to evaluate the simultaneous use of two drugs, we previously have confirmed the synergism of vincristine and prednisolone cytotoxicity against lymphoid cells. Experiments were now carried out to determine whether other steroid hormones can be substituted for prednisolone. Partial or complete potentiation of vincristine cytotoxicity comparable to that achieved by the addition of prednisolone was observed when the latter drug was replaced by a variety of mineralocorticoids (aldosterone, desoxycorticosterone and fludrocortisone), glucocorticoids (hydrocortisone, dexamethasone), androgens (testosterone, methyltestosterone, androsterone, dehydroepiandrosterone, etiocholanolone), estrogens (estrone, 17-beta estradiol) and progestins (progesterone, pregnanediol). No potentiation of cytotoxicity was observed when nonsteroidal hormones (thyroxin, ACTH) were added to vincristine. It is concluded that a wide variety of compounds with the basic perhydrocyclopentanophenanthrene nucleus of the steroid molecule are capable of enhancing the cytotoxicity achieved with vincristine.
利用体外系统评估两种药物的同时使用情况，我们之前已证实长春新碱和泼尼松龙对淋巴细胞的细胞毒性具有协同作用。现在进行实验以确定是否可用其他甾体激素替代泼尼松龙。当用多种盐皮质激素（醛固酮、脱氧皮质酮和氟氢可的松）、糖皮质激素（氢化可的松、地塞米松）、雄激素（睾酮、甲基睾酮、雄酮、脱氢表雄酮、本胆烷醇酮）、雌激素（雌酮、17-β-雌二醇）和孕激素（孕酮、孕二醇）替代泼尼松龙时，观察到长春新碱细胞毒性的部分或完全增强，其增强程度与添加泼尼松龙时相当。当向长春新碱中添加非甾体激素（甲状腺素、促肾上腺皮质激素）时，未观察到细胞毒性增强。结论是，具有甾体分子基本全氢环戊烷并菲核的多种化合物能够增强长春新碱所产生的细胞毒性。
