Vogiatzis A, Marshall G E, Konstas A G, Lee W R
Tennent Institute of Ophthalmology, Glasgow.
Br J Ophthalmol. 1994 Nov;78(11):850-8. doi: 10.1136/bjo.78.11.850.
The present investigation was undertaken to determine if some of the components of exfoliation material in iris tissue were unique to exfoliation or were part of normal iris architecture. Eleven normal iris specimens and 10 exfoliative iris specimens were processed for cryoultramicrotomy and London resin white embedding. Immunogold electron microscopy was used to investigate the fine structural distribution of amyloid P component, elastin, entactin, fibronectin, gp115, and vitronectin in normal iris and their association with exfoliation material. Exfoliation material was positive for amyloid P component and possibly gp115, neither of which were present in normal iris tissue. Elastin and fibronectin were present in the normal iris stroma but were not associated with exfoliation material. The distribution of amyloid P component in the vessel lumen and wall led to the conclusion that amyloid P is a serum contaminant. The presence of gp115 in exfoliation material represents the synthesis of a component novel to the iris vascular cell synthetic repertoire.
本研究旨在确定虹膜组织中剥脱物质的某些成分是剥脱所特有的，还是正常虹膜结构的一部分。对11个正常虹膜标本和10个剥脱性虹膜标本进行了冷冻超薄切片和伦敦树脂白包埋处理。采用免疫金电子显微镜研究淀粉样蛋白P成分、弹性蛋白、巢蛋白、纤连蛋白、gp115和玻连蛋白在正常虹膜中的精细结构分布及其与剥脱物质的关系。剥脱物质对淀粉样蛋白P成分呈阳性反应，可能对gp115也呈阳性反应，而这两种物质在正常虹膜组织中均不存在。弹性蛋白和纤连蛋白存在于正常虹膜基质中，但与剥脱物质无关。淀粉样蛋白P成分在血管腔和血管壁中的分布得出结论，淀粉样蛋白P是一种血清污染物。gp115在剥脱物质中的存在代表了虹膜血管细胞合成库中一种新成分的合成。
