O'Boyle K P, Wright K
Montefiore Medical Center/Albert Einstein Cancer Center, Bronx, New York 10467.
Hum Antibodies Hybridomas. 1994;5(1-2):25-31.
Partially desialylated ovine submaxillary mucin plus an immunological adjuvant has been used by us to induce a humoral immune response to Tn antigen and sialylated Tn in colon cancer patients at risk of recurrence. Peripheral blood lymphocytes were purified from one of these patients with a high IgM titer reactive with Tn antigen transformed with Epstein-Barr virus and subsequently fused with a human-mouse heteromyeloma cell line, HMMA2.11TG/O. Clones were screened and subcloned using an enzyme-linked immunoassay and four stable IgM-secreting clones were tested for reactivity with a variety of natural and synthetic antigens, paraffin-embedded colon carcinomas and normal colonic mucosa to determine their specificity. Four human IgM monoclonal antibodies reactive predominantly with Tn antigen were produced and shown to react with a mucinous human colon carcinoma cell line, LS-174T, and with paraffin-embedded human colon carcinomas. We conclude that modified ovine submaxillary mucin is an effective vaccine for generating a humoral immune response directed to Tn antigen present on human colon cancer.
我们已使用部分去唾液酸化的绵羊颌下粘蛋白加免疫佐剂，对有复发风险的结肠癌患者诱导针对Tn抗原和唾液酸化Tn的体液免疫反应。从其中一名对Tn抗原有高IgM滴度反应的患者中纯化外周血淋巴细胞，用爱泼斯坦-巴尔病毒转化，随后与人类-小鼠杂种骨髓瘤细胞系HMMA2.11TG/O融合。使用酶联免疫测定法筛选并亚克隆克隆，测试四个稳定分泌IgM的克隆与多种天然和合成抗原、石蜡包埋的结肠癌和正常结肠黏膜的反应性，以确定它们的特异性。产生了四种主要与Tn抗原反应的人IgM单克隆抗体，显示它们与粘液性人结肠癌细胞系LS-174T以及石蜡包埋的人结肠癌发生反应。我们得出结论，修饰的绵羊颌下粘蛋白是一种有效的疫苗，可产生针对人结肠癌上存在的Tn抗原的体液免疫反应。
