O'Boyle K P, Markowitz A L, Khorshidi M, Lalezari P, Longenecker B M, Lloyd K O, Welt S, Wright K E
Montefiore Medical Center/Albert Einstein Cancer Center, Bronx, New York, USA.
Hybridoma. 1996 Dec;15(6):401-8. doi: 10.1089/hyb.1996.15.401.
T, Tn, and sialyated Tn (sTn) are pancarcinoma antigens, and increased expression of these carbohydrate epitopes has been correlated with a poor prognosis in several epithelial malignancies. Ten murine monoclonal antibodies have been generated to these antigens, and compared by ELISA and immunohistochemistry to established mAbs reactive with these antigens. Nine mAbs (3 IgM and 6 IgG) reactive with synthetic T-human serum albumin (T-HSA) were produced after immunizing BALB/c mice with a synthetic T-keyhole limpet hemocyanin glycoconjugate (T-KLH). An additional IgM mAb (145.22) was produced in mice immunized with erythrocytes isolated from a patient with Tn syndrome. Three IgM and six IgG1 mAbs reactive with T-HSA did not react with natural T antigen present on desialyated glycophorin. All three IgM and several IgG1 mAbs, however, did react with LS-174T, a mucinous colon carcinoma cell line, 647V, a human bladder carcinoma cell line, and TA3Ha, a murine mammary carcinoma cell line as well as fresh frozen colon carcinomas. MAb 145.22 reacted with both natural and synthetic sources of sTn and Tn, as well as with LS-174T cells and mucin deposits in 10/11 colon carcinomas on fresh-frozen sections. MAb B72.3 reacted strongly with ovine submaxillary mucin (OSM) and sTn-HSA, while mAb CC49, a second-generation mAb to TAG-72 carcinoma mucin, reacted strongly with OSM, less strongly with desialyated OSM, and only weakly with sTn-HSA, suggesting that the epitope specificity for mAb CC49 is distinct from that of B72.3.
T抗原、Tn抗原和唾液酸化Tn抗原（sTn）是泛癌抗原，在几种上皮性恶性肿瘤中，这些碳水化合物表位的表达增加与预后不良相关。已针对这些抗原产生了十种鼠单克隆抗体，并通过酶联免疫吸附测定（ELISA）和免疫组织化学与已确立的针对这些抗原的单克隆抗体进行比较。用合成的T-钥孔戚血蓝蛋白糖缀合物（T-KLH）免疫BALB/c小鼠后，产生了九种与合成的T-人血清白蛋白（T-HSA）反应的单克隆抗体（3种IgM和6种IgG）。在用从一名Tn综合征患者分离的红细胞免疫的小鼠中产生了另外一种IgM单克隆抗体（145.22）。三种与T-HSA反应的IgM和六种IgG1单克隆抗体不与去唾液酸化血型糖蛋白上存在的天然T抗原发生反应。然而，所有三种IgM和几种IgG1单克隆抗体确实与黏液性结肠癌细胞系LS-174T、人膀胱癌细胞系647V、鼠乳腺癌细胞系TA3Ha以及新鲜冷冻的结肠癌发生反应。单克隆抗体145.22与sTn和Tn的天然及合成来源以及新鲜冷冻切片上10/11例结肠癌中的LS-174T细胞和黏蛋白沉积物发生反应。单克隆抗体B72.3与羊颌下黏蛋白（OSM）和sTn-HSA强烈反应，而针对TAG-72癌黏蛋白的第二代单克隆抗体CC49与OSM强烈反应，与去唾液酸化的OSM反应较弱，与sTn-HSA仅微弱反应，这表明单克隆抗体CC49的表位特异性与B72.3不同。
