Krallmann-Wenzel U, Schmidt G
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin.
Zentralbl Bakteriol. 1994 Aug;281(2):140-5. doi: 10.1016/s0934-8840(11)80564-5.
Enteropathogenic Escherichia coli O111 bacteria very often produce a capsule-like polysaccharide exhibiting the same chemical composition as the O-specific chains of their lipopolysaccharide and being designated as O-antigen capsule. In this paper, a new simple procedure is described for the detection of this capsule polymer. O-antigen capsule polysaccharide and lipopolysaccharide of bacterial extracts were electrophoretically separated in agarose cetavlon gel, blotted onto nitrocellulose membranes and visualized by immunological methods. As a result of this procedure, the O-antigen capsule can be detected as a spot completely separated from the LPS (Figs. 1 and 2). Consequently, this method allows a clear discrimination of K+ and K- strains.
肠道致病性大肠杆菌O111常常产生一种类似荚膜的多糖，其化学组成与脂多糖的O特异性链相同，被称为O抗原荚膜。本文描述了一种检测这种荚膜聚合物的新的简单方法。细菌提取物中的O抗原荚膜多糖和脂多糖在琼脂糖十六烷基三甲基溴化铵凝胶中进行电泳分离，转移到硝酸纤维素膜上，并用免疫方法进行显色。通过该方法，O抗原荚膜可被检测为一个与脂多糖完全分离的斑点（图1和图2）。因此，该方法能够清晰地区分K+和K-菌株。
