使用伊红Y染色法检测聚丙烯酰胺凝胶中的蛋白质和唾液酸糖蛋白。
Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain.
作者信息
Lin F, Wise G E
机构信息
Department of Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
出版信息
Mol Biotechnol. 1994 Apr;1(2):147-50. doi: 10.1007/BF02921555.
PMID:7532101
Abstract
An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.
摘要
本文描述了一种曙红Y染色技术，该技术可用于检测聚丙烯酰胺凝胶中的各种蛋白质，包括膜唾液糖蛋白。曙红Y染色法的灵敏度与银染色相当。此外，该方法还有一个额外的优点，即染色后的蛋白质在蛋白质印迹法中仍保留抗原性。文中还介绍了获得最佳染色结果的操作细节。
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