Lin F, Fan W, Wise G E
Department of Anatomy and Cell Biology, Texas College of Osteopathic Medicine/University of North Texas, Fort Worth 76107.
Anal Biochem. 1991 Aug 1;196(2):279-83. doi: 10.1016/0003-2697(91)90466-7.
A staining method is described in which various proteins in polyacrylamide gels can be stained by using eosin Y. After a brief incubation of a polyacrylamide gel in an acidic solution of 1% eosin Y, various proteins, including human erythrocyte membrane sialoglycoproteins which are not detectable by Coomassie blue R-250 (CB), can be detected with a sensitivity of 10 ng protein. This is far more sensitive than CB staining and is comparable to the sensitivity of silver staining. In a Western blot, the antigenicity of an eosin Y stained protein is retained. In addition, proteins on an immunoblot sheet can be detected by eosin Y staining. The method described is rapid, sensitive, and reproducible with various proteins in polyacrylamide gels and has the added advantage of also staining sialoglycoproteins.
本文描述了一种染色方法，通过该方法可使用伊红Y对聚丙烯酰胺凝胶中的多种蛋白质进行染色。将聚丙烯酰胺凝胶在1%伊红Y的酸性溶液中短暂孵育后，包括考马斯亮蓝R-250（CB）无法检测到的人红细胞膜唾液酸糖蛋白在内的多种蛋白质，均可被检测到，其灵敏度可达10 ng蛋白质。这比CB染色灵敏得多，与银染色的灵敏度相当。在蛋白质印迹法中，伊红Y染色的蛋白质抗原性得以保留。此外，免疫印迹膜上的蛋白质也可用伊红Y染色进行检测。所描述的方法快速、灵敏，对聚丙烯酰胺凝胶中的各种蛋白质具有可重复性，并且还具有能对唾液酸糖蛋白进行染色的额外优势。
