Abernathy-Carver K J, Sampson H A, Picker L J, Leung D Y
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
J Clin Invest. 1995 Feb;95(2):913-8. doi: 10.1172/JCI117743.
The extravasation of T cells at sites of inflammation is critically dependent on the activity of homing receptors (HR) involved in endothelial cell recognition and binding. Two such HR (the cutaneous lymphocyte antigen [CLA] and L-selectin) have been shown to be selectively involved in T cell migration to skin and peripheral lymph nodes, respectively. This study was designed to assess the relationship between the organ specificity of an allergic reaction to food and the expression of HR on T cells activated in vitro by the relevant food allergen. Peripheral blood mononuclear cells were isolated from seven milk allergic children with a history of eczema when exposed to milk. All patients had a positive prick skin test and double-blind placebo-controlled food challenge to milk. 10 children with either allergic eosinophilic gastroenteritis or milk-induced enterocolitis and 8 nonatopic adults served as controls. Five-parameter flow cytometry using monoclonal antibodies was used for detection of the specific HR on freshly isolated T cells versus T cell blasts induced by a 6-d incubation with casein, as compared with Candida albicans. After in vitro stimulation with casein, but not C. albicans, patients with milk allergy and atopic dermatitis had a significantly greater percentage of CLA+ T cells (P < 0.01) than controls with milk-induced enterocolitis, allergic eosinophilic gastroenteritis, or nonatopic healthy controls. In contrast, the percentage of L-selectin-expressing T cells did not differ significantly between these groups. These data suggest that after casein stimulation allergic patients with milk-induced skin disease have an expanded population of CLA+ T cells, as compared with nonatopics or allergic patients without skin involvement. We postulate that heterogeneity in the regulation of HR expression on antigen-specific T cells may play a role in determining sites of involvement in tissue-directed allergic responses.
T细胞在炎症部位的外渗严重依赖于参与内皮细胞识别和结合的归巢受体（HR）的活性。已证明两种这样的HR（皮肤淋巴细胞抗原[CLA]和L-选择素）分别选择性地参与T细胞向皮肤和外周淋巴结的迁移。本研究旨在评估食物过敏反应的器官特异性与相关食物过敏原体外激活的T细胞上HR表达之间的关系。从7名有牛奶过敏且有湿疹病史的儿童中分离外周血单核细胞，这些儿童在接触牛奶时会出现过敏反应。所有患者对牛奶的皮肤点刺试验和双盲安慰剂对照食物激发试验均呈阳性。10名患有过敏性嗜酸性胃肠炎或牛奶诱导的小肠结肠炎的儿童和8名非特应性成年人作为对照。使用单克隆抗体的五参数流式细胞术用于检测新鲜分离的T细胞与经酪蛋白孵育6天诱导的T细胞母细胞上的特异性HR，并与白色念珠菌进行比较。在用酪蛋白而非白色念珠菌进行体外刺激后，牛奶过敏和特应性皮炎患者的CLA+T细胞百分比显著高于牛奶诱导的小肠结肠炎、过敏性嗜酸性胃肠炎患者或非特应性健康对照（P<0.01）。相比之下，这些组中表达L-选择素的T细胞百分比没有显著差异。这些数据表明，与非特应性患者或无皮肤受累的过敏患者相比，酪蛋白刺激后患有牛奶诱导性皮肤病的过敏患者中CLA+T细胞群体有所扩大。我们推测抗原特异性T细胞上HR表达调控的异质性可能在决定组织定向过敏反应的受累部位中起作用。
