Tiitta O, Happonen R P, Virtanen I, Luomanen M
Department of Anatomy, University of Helsinki, Finland.
J Oral Pathol Med. 1994 Nov;23(10):446-50. doi: 10.1111/j.1600-0714.1994.tb00442.x.
The distribution of the extracellular matrix (ECM) protein tenascin (Tn) was studied in oral premalignant lesions and squamous cell carcinoma by using the monoclonal antibody (Mab) 143DB7. In normal buccal and palatal mucosa, in ventral tongue, in floor of mouth and in gingiva, immunoreaction for Tn was seen to be distributed as a continuous thin, delicate line merely in the basement membrane region. Hyperkeratosis without dysplasia showed a distinct zone of enhanced Tn immunoreactivity immediately beneath the epithelium. In dysplasias of various degree, enhancement of the stromal Tn content could be observed, being most conspicuous in carcinoma in situ lesions. In most invasive carcinoma cases the Tn immunoreactivity was intense, extending deeply into the underlying stroma. In such lesions Tn-reaction often covered the total stroma. Notably, the strongest immunoreaction was seen at the advancing edges of the tumor. The triggering factor for stromal Tn enhancement seems to be of epithelial origin. The enhanced expression of Tn suggests that Tn plays a role in organizing and remodelling the stroma to support active epithelial proliferation and migration. However, it also seems that inflammation is associated with Tn expressions.
采用单克隆抗体（Mab）143DB7研究了细胞外基质（ECM）蛋白腱生蛋白（Tn）在口腔癌前病变和鳞状细胞癌中的分布。在正常颊黏膜和腭黏膜、舌腹、口底及牙龈中，Tn的免疫反应仅在基底膜区域呈连续的细薄、精致线条分布。无发育异常的角化过度在紧邻上皮下方显示出明显增强的Tn免疫反应区。在不同程度的发育异常中，可观察到基质中Tn含量增加，原位癌病变中最为明显。在大多数浸润性癌病例中，Tn免疫反应强烈，深入下方基质。在这类病变中，Tn反应常覆盖整个基质。值得注意的是，在肿瘤前沿可见最强的免疫反应。基质中Tn增强的触发因素似乎源于上皮。Tn表达增强表明Tn在组织和重塑基质以支持上皮细胞的活跃增殖和迁移中发挥作用。然而，炎症似乎也与Tn表达有关。
