RNA螺旋中的嘌呤-嘌呤错配：质子化G·A碱基对及次近邻效应的证据
Purine-purine mismatches in RNA helices: evidence for protonated G.A pairs and next-nearest neighbor effects.
作者信息
Morse S E, Draper D E
机构信息
Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218.
出版信息
Nucleic Acids Res. 1995 Jan 25;23(2):302-6. doi: 10.1093/nar/23.2.302.
Abstract
Thermodynamic parameters are presented for 12 different RNA duplexes containing A.A, A.G, G.A and G.G mismatches flanked by C-G base pairs. UV melting studies were conducted under three different buffer conditions in order to evaluate the effects of salt concentration and pH on the stability of each mismatch-containing duplex. The main findings are: (i) the mismatches have a wide range of effects on duplex stability, decreasing delta G degrees 37 of denaturation by approximately 0-7 kcal/mol; (ii) the nearest-neighbor assumption commonly used to calculate helix stability breaks down for G.A mismatches; and (iii) G.A mismatches separated by 2 bp form a protonated structure.
摘要
给出了12种不同RNA双链体的热力学参数，这些双链体包含两侧为C-G碱基对的A·A、A·G、G·A和G·G错配。为了评估盐浓度和pH对每个含错配双链体稳定性的影响，在三种不同缓冲条件下进行了紫外熔解研究。主要发现如下：(i)错配对双链体稳定性有广泛影响，使变性的ΔG°37降低约0 - 7千卡/摩尔；(ii)通常用于计算螺旋稳定性的最近邻假设对于G·A错配不成立；(iii)被2个碱基对隔开的G·A错配形成质子化结构。
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